...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Agricultural and Food Chemistry >Improvement of a P450-Based Recombinant Escherichia coli Whole-Cell System for the Production of Oxygenated Sesquiterpene Derivatives
【24h】

Improvement of a P450-Based Recombinant Escherichia coli Whole-Cell System for the Production of Oxygenated Sesquiterpene Derivatives

机译：基于P450的重组大肠杆菌整体细胞系统的改进，用于生产含氧倍霉素衍生物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sesquiterpenes are common constituents of essential oil in plants. Their oxygenated derivatives often possess desirable flavor, fragrance, and pharmaceutical properties. Recently, the CYP264B1-based recombinant Escherichia coli whole-cell system has been constructed for the oxidation of sesquiterpenes. However, limiting factors of this system related to the high volatility of substrates and the suitability of the P450 redox partner need to be addressed. In this work, the improvement of the system was implemented with (+)-alpha-longipinene as a model substrate. By using, 2-hydroxypropyl-beta-cyclodextrin and an alternative ferredoxin reductase, the conversion of (+)-alpha-longipinene was improved 77.1%. Applying the optimized conditions, the yields of the main products were 54.2, 34.2, and 47.2 mg L-1, corresponding to efficiencies of 82.1, 51.8, and 71.5% for the conversion of (+)-alpha-longipinene, (-)-isolongifolene, and alpha-humulene, respectively, at a 200 mL scale. These products were characterized as 12-hydroxy-alpha-longipinene, isolongifolene-9-one, and 5-hydroxy-alpha-humulene, respectively, by nuclear magnetic resonance spectroscopy.
机译：Sesquiterpenes是植物中精油的常见成分。它们的含氧衍生物通常具有所需的风味，香味和药物性质。最近，已构建基于CYP264B1的重组大肠杆菌的COLI全细胞系统用于筛分氧化萜烯。然而，该系统的限制因素与基材的高挥发性和P450氧化还原伴侣的适用性有关的系统需要解决。在这项工作中，系统的改进用（+） - α-少ineine作为模型基质。通过使用2-羟丙基 - β-环糊精和替代的福兰辛还原酶，（+） - α-稀丙烯烯的转化率提高了77.1％。应用优化条件，主要产物的产量为54.2,34.2和47.2mg L-1，对应于（+） - α-少ineNe，（ - ）转化的82.1,51.8和71.5％的效率 - 分离性二醇和α-海豚，分别为200毫升规模。这些产物分别通过核磁共振光谱分别以核磁共振光谱分别为12-羟基 - α-少茚，isolongifolene-9-α和5-羟基 - α-冲突。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号