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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Potential Lipid-Lowering Mechanisms of Biochanin A
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Potential Lipid-Lowering Mechanisms of Biochanin A

机译：生物脉素A的潜在降脂机制

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Extensive studies have demonstrated that biochanin A (BCA) has a significant hypolipidemic effect. However, its mechanism of action is not clear. In this context, the effect of BCA on a high-fat diet (HFD)-induced hyperlipidemia in mice was determined. The results showed that treatment with a medium dose of biochanin A (BM) significantly decreased low-density lipoprotein cholesterol (LDL-C) 85% (from 1.196 +/- 0.183 to 0.181 +/- 0.0778 mM) and total cholesterol (TC) 39% (from 5.983 +/- 0.128 to 3.649 +/- 0.374 mM) levels, increased lipoprotein lipase (LPL) 96% (from 1.421 +/- 0.0982 to 2.784 +/- 0.177 U/mg protein) and hepatic triglyceride lipase (HTGL) 78% (from 1.614 +/- 0.0848 to 2.870 +/- 0.0977 U/mg protein) activities, significantly improved fecal lipid levels, and lowered the epididymal fat index in hyperlipidemic mice compared with the HFD control mice (p < 0.05). In vitro, the high antioxidant capacity of BCA was determined by the FRAP assay, ABTS(center dot+) scavenging method, and an ROS assay. In RAW 264.7 macrophages, a dose of 10 mu M BCA significantly increased the cholesterol efflux by 18.7% compared with the control cells. Moreover, molecular docking of BCA on cholesterol ester transfer protein (CETP) (Asn24 and Thr27 at the N-terminal; A1a274 and Phe270 at the C-terminal) gave new insights into the role of BCA in preventing cholesterol ester transport.
机译：广泛的研究表明，生物脉素A（BCA）具有显着的低血脂效应。但是，它的行动机制尚不清楚。在这种情况下，测定BCA对高脂饮食（HFD） - 诱导小鼠的高脂血症的影响。结果表明，用培养基剂量的生物脉素A（BM）处理显着降低了低密度脂蛋白胆固醇（LDL-C）85％（从1.196 +/- 0.183至0.181 +/- 0.0778mm）和总胆固醇（Tc） 39％（从5.983 +/- 0.128至3.649 +/- 0.374 mm）水平，增加脂蛋白脂肪酶（LPL）96％（从1.421 +/- 0.0982至2.784 +/- 0.177 U / mg蛋白）和肝甘油三酯脂肪酶（ HTGL）78％（从1.614 +/- 0.0848至2.870 +/- 0.0977 U / mg蛋白）的活动，显着改善粪便脂质水平，并与HFD对照小鼠相比降低了高脂血小胺小鼠的附睾脂肪指数（P <0.05） 。体外，通过FRAP测定，ABTS（中心点+）清除方法和ROS测定法测定BCA的高抗氧化能力。在Raw 264.7巨噬细胞中，与对照细胞相比，10μmBCa的剂量显着增加18.7％的胆固醇渗透。此外，BCA对胆固醇酯转移蛋白（CETP）（ASN24和N-末端的ASN24和THR27的分子对接; C-末端的A1A274和PHE270）对BCA在预防胆固醇酯转运方面的作用进行了新的见解。

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