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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Flavanol Quantification of Grapes via Multiple Reaction Monitoring Mass Spectrometry. Application to Differentiation among Clones of Vitis vinifera L. cv. Rufete Grapes
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Flavanol Quantification of Grapes via Multiple Reaction Monitoring Mass Spectrometry. Application to Differentiation among Clones of Vitis vinifera L. cv. Rufete Grapes

机译：通过多重反应监测质谱法的黄烷醇量化葡萄。 在 vInifera L.CV中的克隆中的施用施用。 Rufete葡萄

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src="http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-31/acs.jafc.6b05278/20170803/images/medium/jf-2016-05278s_0004.gif">The determination of the detailed flavanol composition in food matrices is not a simple task because of the structural similarities of monomers and, consequently, oligomers and polymers. The aim of this study was the development and validation of an HPLC-MS/MS–multiple reaction monitoring (MRM) method that would allow the accurate and precise quantification of catechins, gallocatechins, and oligomeric proanthocyanidins. The high correlation coefficients of the calibration curves (>0.993), the recoveries not statistically different from 100%, the good intra- and interday precisions (<5%), and the LOD and LOQ values, low enough to quantify flavanols in grapes, are good results from the method validation procedure. Its usefulness has also been tested by determining the detailed composition of Vitis vinifera L. cv. Rufete grapes. Seventy-two (38 nongalloylated and 34 galloylated) and 53 (24 procyanidins and 29 prodelphinidins) flavanols have been identified and quantified in grape seed and grape skin, respectively. The use of HCA and PCA on the detailed flavanol composition has allowed differentiation among Rufete clones.
机译：src =“http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-31/acs.jafc.6b05278/20170803/images/medium / jf-2016-05278s_0004.gif">食物基质中的详细黄烷醇组合物的测定不是一个单体的结构相似性，因此，低聚物和聚合物是一种简单的任务。该研究的目的是HPLC-MS / MS多反应监测（MRM）方法的开发和验证，其将允许足够精确地定量儿茶素，加洛伐克和低聚原花青素。校准曲线的高相关系数（> 0.993），回收率与100％没有统计学不同，良好的内部和白天矫反比（<5％），以及LOD和LOQ值，足以量化葡萄中的黄黄醇，来自方法验证程序是好的结果。通过确定血管血管血管 L.CV的详细组成，还测试了其有用性。 rufete葡萄。七十二（38种Nongallylated和34 allabroylated）和53（24种ProAnyanidins和29个Phorlphinidins）已经鉴定并分别在葡萄籽和葡萄皮中定量。 HCA和PCA在详细的黄烷醇组合物上的使用在Rufete克隆中允许分化。

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