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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Enhancement of Naringenin Biosynthesis from Tyrosine by Metabolic Engineering of Saccharomyces cerevisiae
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Enhancement of Naringenin Biosynthesis from Tyrosine by Metabolic Engineering of Saccharomyces cerevisiae

机译：通过酿酒酵母的代谢工程，从酪氨酸增强芽孢菌素生物合成的

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src="http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-31/acs.jafc.7b02507/20170803/images/medium/jf-2017-02507u_0005.gif">Flavonoids are an important class of plant polyphenols that possess a variety of health benefits. In this work, S. cerevisiae was metabolically engineered to produce the flavonoid naringenin, using tyrosine as the precursor. Our strategy to improve naringenin production comprised three modules. In module 1, we employed a modified GAL system to overexpress the genes of the naringenin biosynthesis pathway and investigated their synergistic action. In module 2, we simultaneously up-regulated acetyl-CoA production and down-regulated fatty acid biosynthesis in order to increase the precursor supply, malonyl-CoA. In module 3, we engineered the tyrosine biosynthetic pathway to eliminate the feedback inhibition of tyrosine and also down-regulated competing pathways. It was found that modules 1 and 3 played important roles in improving naringenin production. We succeeded in producing up to ?90 mg/L of naringenin in our final strain, which is a 20-fold increase as compared to the parental strain.
机译：src =“http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-31/acs.jafc.7b0203/20170803/images/medium / jf-2017-02507u_0005.gif"clavonoids是具有各种健康益处的重要植物多酚。在这项工作中， s。酿酒酵母（Cerevisiae）在使用酪氨酸作为前体的前体代谢地改造以产生类黄酮鼻腔蛋白。我们改善柚皮素生产的策略包括三个模块。在模块1中，我们使用修饰的 GAL 系统来过度表达Naringenin生物合成途径的基因，并研究了它们的协同作用。在模块2中，我们同时调节乙酰基辅菌生产和下调脂肪酸生物合成，以增加前体供应，丙二酰库。在模块3中，我们设计了酪氨酸生物合成途径，以消除酪氨酸的反馈抑制，以及下调的竞争途径。结果发现，模块1和3在改善柚皮素生产方面发挥了重要作用。在我们的最终菌株中，我们成功地产生了90毫克/升的柚皮素，与亲本菌株相比，这是20倍的增加。

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  • 作者

    Xiaomei Lyu; Kuan Rei Ng; Jie Lin Lee; Rita Mark; Wei Ning Chen;

  • 作者单位

    School of Chemical and Biomedical Engineering College of Engineering Nanyang Technological University 62 Nanyang Drive Singapore 637459 Singapore;

    School of Chemical and Biomedical Engineering College of Engineering Nanyang Technological University 62 Nanyang Drive Singapore 637459 Singapore;

    School of Chemical and Biomedical Engineering College of Engineering Nanyang Technological University 62 Nanyang Drive Singapore 637459 Singapore;

    School of Chemical and Biomedical Engineering College of Engineering Nanyang Technological University 62 Nanyang Drive Singapore 637459 Singapore;

    School of Chemical and Biomedical Engineering College of Engineering Nanyang Technological University 62 Nanyang Drive Singapore 637459 Singapore;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 营养卫生、食品卫生;农业科学;
  • 关键词

    metabolic engineering; naringenin; lt; igt; Saccharomyces cerevisiaelt; /igt; tyrosine;

    机译：代谢工程;Naringenin;＆lt;I＆gt;酿酒酵母酿酒酵母;酪氨酸;

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