• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Applied Polymer Science >Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture
【24h】

Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture

机译：聚合物大孔矩阵中的糖固定中的增强性捕获

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The use of macroporous monolithic matrices in the purification of biocompounds is constantly growing and developing. In this work, the objective was to optimize the quantity of N-acetyl-D-glucosamine (D-GlcNAc) immobilized on the surface of macroporous polymeric cryogels for capture of lectins from less clarified solutions. Surface response methodology was applied and it was observed that the immobilization temperature of the glutaraldehyde (GLU) and the D-GlcNAc concentration influenced the amount of sugar immobilized. The matrices produced with 1.1% of allyl glycidyl ether were functionalized by GLU. Optimal maximum condition was obtained with mean value of 160.39 +/- 26.38 mg of D-GlcNAc immobilized per gram of dry cryogel. Characterization analyses of the matrices showed that the activation process was effective, maintaining the macroporous structure and physical characteristics. The adsorbents produced were tested for capture of lectins from a crude protein solution of barley. At tested conditions, adsorbent capture around 11% of protein in solution but reduce the hemaglutinating capacity in 40%, demonstrating its selectivity. The cryogels functionalized with D-GlcNAc present potential for use in capture compounds by affinity with carbohydrates, such as lectins. (c) 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47956.
机译：在biocompounds的纯化使用大孔单片矩阵的不断成长和发展。在这项工作中，其目的是优化用于固定的凝集素捕获大孔聚合物冷冻凝胶的表面上从以下澄清溶液N-乙酰基d葡糖胺（d-GlcNAc的）的量。表面响应方法被应用，且观察到戊二醛（GLU）和d-GlcNAc的浓度的固定化温度的影响糖的固定化量。产生具有的烯丙基缩水甘油醚通过GLU官能1.1％的基质。用的160.39 +/- 26.38毫克d-GlcNAc的的平均值获得的最佳最大条件每克干冻凝胶的固定化。矩阵表征的分析表明，激活过程是有效的，保持大孔结构和物理特性。产生的吸附剂中测试凝集素捕获来自大麦的粗蛋白质溶液。在所测试的条件下，在周围溶液的蛋白质的11％吸附剂捕获但减少40％hemaglutinating容量，表明其选择性。与用于通过与碳水化合物，如外源凝集素亲和捕获中使用的化合物d-GlcNAc的本潜在官能的冷冻凝胶。 （c）2019 Wiley期刊，Inc.J.Phill。聚合物。 SCI。 2019年，136，47956。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号