• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Applied Polymer Science >Preparation of a modified crosslinked chitosan/polyvinyl alcohol blended affinity membrane for purification of His-tagged protein
【24h】

Preparation of a modified crosslinked chitosan/polyvinyl alcohol blended affinity membrane for purification of His-tagged protein

机译：制备改性交联的壳聚糖/聚乙烯醇混合亲和膜，用于纯化His标记的蛋白质

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Based on a crosslinked chitosan (CS)/polyvinyl alcohol (PVA) matrix membrane, an immobilized metal ion affinity membrane (IMAM) using Cu2+ and Ni2+ ions as affinity ligands was prepared for purification of the His-tagged recombinant protein. The affinity membrane possessed a favorable membrane structure including 1.39 mu m average pore size and 0.33 mL.cm(-2).s(-1) water flux under 0.08 MPa pressure at 25 degrees C. The Cu2+ and Ni2+ ions capacities immobilized on the IMAM were 155.6 and 137.3 mu mol.disk(-1), respectively. The IMAM had an excellent specific affinity to His-tagged protein. About 10-fold purification factor for the model protein was obtained in a batch adsorption, and serine hydroxymethyl transferase could be purified to a single band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis from its crude extract solution with an affinity membrane cartridge by a dynamic purification process. This work provides a promising IMAM for the purification of His-tagged recombinant proteins. (C) 2018 Wiley Periodicals, Inc.
机译：基于交联脱乙酰壳多糖（CS）/聚乙烯醇（PVA）基质膜，固定化金属离子亲和膜（IMAM）使用的Cu2 +和Ni2 +离子作为亲和配体是为His-标记的重组蛋白的纯化制备的。亲和膜所具有的良好的膜结构，其包括1.39亩在25℃下的铜离子和Ni2 +离子的能力固定在所述M个下0.08兆帕压力平均孔径和0.33 mL.cm（-2）.S（-1）的水通量IMAM是155.6和137.3亩mol.disk（-1），分别。伊玛目具有极好的特异性亲和力His标签的蛋白质。大约为模型蛋白质10倍的纯化因子在间歇吸附得到，和丝氨酸羟甲基转移酶可以由动态纯化以单一条带在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析来自与亲和膜滤芯的粗提取物溶液纯化过程。这项工作提供了他的标签的重组蛋白的纯化一个有前途的伊玛目。 （c）2018 Wiley期刊，Inc。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号