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Highly porous protein-based 3D scaffolds with different collagen concentrates for potential application in tissue engineering

机译:具有不同胶原蛋白的基于高孔蛋白的3D支架,用于组织工程中的潜在应用

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Different researchers have developed protein-based scaffolds for tissue engineering, although few of them have combined their development with previous characterization of the raw material used. The main objective of this work was to characterize four Type I collagen protein concentrates from different sources (pork HI95, pork T95, turkey, and fish) and study their use as scaffolds for tissue engineering. Thus, the chemical and amino acid composition, protein solubility, and Z-potential of the different concentrates were analyzed in the pH range between 2 and 12. In addition, the mechanical and structural properties of the different scaffolds were compared. The initial characterization of the four concentrates shows similarities, such as a typical amino acid profile and an isoelectric point between pH 5 and 6. In fact, differences based on composition, namely, systems with higher protein concentration, results in more deformable scaffolds, whereas the ones processed from fish collagen, with a higher glycine percentage, shows the highest porosity and pore sizes. (c) 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47954.
机译:不同的研究人员已经开发出基于蛋白质的组织工程支架,尽管它们中的很少有利用所使用的原材料的先前表征结合了他们的发展。这项工作的主要目标是表征来自不同来源的四种I型胶原蛋白浓缩物(猪肉HI95,猪肉T95,土耳其和鱼类),并研究它们用作组织工程的支架。因此,在第2和12之间的pH范围内分析了不同浓缩物的化学和氨基酸组成,蛋白质溶解度和Z-电位。此外,比较了不同支架的机械和结构性质。四种浓缩物的初始表征显示相似之处,例如典型的氨基酸分布和pH 5和6之间的等电点。实际上,基于组合物的差异,即蛋白质浓度较高的系统,导致更可变形的支架,而是导致更可变形的支架,而且导致更可变形的支架,而且从鱼胶原蛋白加工的那些具有较高的甘氨酸百分比,显示出最高的孔隙率和孔径。 (c)2019 Wiley期刊,Inc.J.Phill。聚合物。 SCI。 2019,136,47954。

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