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首页> 外文期刊>Journal of Applied Polymer Science >Assessment of the ability of poly(l-lysine)-poly(ethylene glycol) (PLL-PEG) hydrogels to support the growth of U87-MG and F98 glioma tumor cells
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Assessment of the ability of poly(l-lysine)-poly(ethylene glycol) (PLL-PEG) hydrogels to support the growth of U87-MG and F98 glioma tumor cells

机译：评估聚（L-赖氨酸） - 聚（乙二醇）（PLL-PEG）水凝胶以支持U87-MG和F98胶质瘤肿瘤细胞生长的能力

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摘要

Biomaterials that provide 3D-like in vitro cell survival and proliferation are increasingly used to mimic the extracellular microenvironment in the context of a better understanding of tumorigenesis. In this study, a simple, affordable and fast technique to fabricate hydrogel matrices composed of poly(ethylene glycol) (PEG) and poly(l-lysine) (PLL) (as cell-adhesive factor) were used to provide in vitro glioma cell growth. After UV photopolymerization of a precursor solution containing PEG-diacrylate and easily obtainable PLL-acrylate derivatives, F98 and U87-MG cells (rat and human glioblastoma cell lines, respectively) were grown on top of different substrates that consist of combinations of PEG/PLL hydrogels and spontaneously formed cell aggregates of homogeneous sizes. Depending on the cell type, PEG and PLL concentrations, the cell aggregates patterns were different. The optimal combination to obtain cell survival and proliferation for both cell lines was determined as 3% PEG (w/v) and 0.001% PLL (w/v). This technique was also used to assess the efficacy of temozolomide and should be adaptable to other cancer cell lines to follow pseudo-tumor growth in vitro. (c) 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46287.
机译：提供3D样的体外细胞存活率和增殖的生物材料越来越多地用于模拟细胞外微环境在更好地理解肿瘤内酯的背景下。在该研究中，使用一种简单，实惠且快速的技术，用于制造由聚（乙二醇）（PEG）和聚（L-赖氨酸）（PLL）（作为细胞粘合剂因子）组成的水凝胶基质来提供体外胶质瘤细胞生长。在含有PEG二丙烯酸酯的前体溶液的UV光聚合之后，在不同的基材上生长含有PEG二丙烯酸酯的前体溶液，F98和U87-Mg细胞（分别）在由PEG / PLL的组合组成的不同基材上生长水凝胶和自发形成均匀尺寸的细胞聚集体。取决于细胞型，PEG和PLL浓度，细胞聚集体模式不同。获得两种细胞系细胞存活和增殖的最佳组合被确定为3％PEG（W / V）和0.001％PLL（W / V）。该技术还用于评估替替莫唑胺的疗效，应适应其他癌细胞系，以遵循体外伪肿瘤生长。 （c）2018 Wiley期刊，Inc.J.Phill。聚合物。 SCI。 2018,135,46287。

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