• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Applied Polymer Science >Guanine oxidation signal enhancement in single strand DNA with polyacrylonitrile/polyaniline (PAN/PAni) hybrid nanofibers
【24h】

Guanine oxidation signal enhancement in single strand DNA with polyacrylonitrile/polyaniline (PAN/PAni) hybrid nanofibers

机译：用聚丙烯腈/聚苯胺（PAN / PANI）杂交纳米纤维单链DNA中的鸟嘌呤氧化信号增强

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pure polyacrylonitrile (PAN) and polyacrylonitrile/polyaniline (PAN/PAni) hybrid nanofibers (NFs) were produced via electrospinning and used to monitor guanine oxidation in single strand DNA (ssDNA) by electrochemical methods. Two different methodologies were conducted. First, pre-synthesized PAni was added into electrospinning PAN solution and electrospun into composite PAN/PAni nanofibrous structure on cylindrical pencil graphite (PGE) surface. In the second route, PAN NFs were electrospun on a PGE surfaces and polymerization of PAni was conducted on the surfaces of the as-spun PAN NFs. NFs were kept at -18 degrees C in a refrigerator for several days. ssDNA was immobilized on the prepared NFs and guanine oxidation signals were observed for each system. The results revealed that use of PAN NFs enhanced signal intensity from 0.92 mu A (PGE) to 1.04 mu A (PAN NFs). Addition of PAni to PAN increased signal intensity to 1.23 mu A. When the PAN NF surfaces were coated with PAni, signal enhancement continued to increase up to 4.19 mu A for fourth day and decreased again when PAni-coated NFs were kept at -18 degrees C in the refrigerator. Since the prepared system is fast and cheap, it is promising for application in DNA biosensor devices. (c) 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45567.
机译：通过静电纺塑制备纯聚丙烯腈（锅）和聚丙烯腈/聚苯胺（PAN / PANI）杂化纳米纤维（NFS），用来通过电化学方法监测单链DNA（SSDNA）中的鸟嘌呤氧化。进行了两种不同的方法。首先，将预合成的PANI加入到​​圆柱形铅笔石墨（PGE）表面上的复合锅/盘纳米纤维结构中的静电纺丝锅溶液和电纺。在第二次途径中，PAN NFS在PGE表面上进行电纺，并在Spun PAN NFS的表面上进行PANI的聚合。 NFS在冰箱中保持在-18℃下几天。将SSDNA固定在制备的NFS上，对每个系统观察到胞氨定氧化信号。结果表明，使用PAN NFS增强信号强度为0.92μA（PGE）至1.04μA（PAN NFS）。将PANI添加到1.23μmA的增加的信号强度增加。当PAN NF表面涂有PANI时，第四天的信号增强继续增加4.19亩，并且当PANI涂覆的NFS保持在-18度时再次下降C在冰箱里。由于制备的系统快速且便宜，因此在DNA生物传感器装置中展示了很有应用。 （c）2017 Wiley期刊，Inc.J.Phill。聚合物。 SCI。 2018,135,45567。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号