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首页> 外文期刊>Journal of Applied Polymer Science >Facile enzymatic preparation of fluorescent conjugated polymers of phenols and their application in sensing
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Facile enzymatic preparation of fluorescent conjugated polymers of phenols and their application in sensing

机译：苯酚荧光共轭聚合物的容易酶促制剂及其在感应中的应用

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The recognized drawback of utilizing metal catalysts for the synthesis of fluorescent conjugated polymers (CP) is the requirement for extensive purification to ensure complete removal of residual catalyst that would otherwise quench the fluorescence. In addition, typical synthesis of fluorescent CP involves multiple steps, monomers and solvents with varying levels of toxicity. This work demonstrates the possibility of utilizing oxidoreductase enzymes as the catalyst, for the one step polymerization of naturally occurring phenols to yield fluorescent conjugated polyphenols. The metal in the active site of the enzyme remains chelated during the synthesis allowing the polymers to be fluorescent as synthesized without the need for extensive purification. Three natural phenols, 4-hydroxyphenylacetic acid, hydroxytyrosol, and chlorogenic acid were polymerized using Horseradish peroxidase as the biocatalyst. Spectroscopic techniques, UV-vis, Fourier transform infrared Spectroscopy-Attenuated Total Reflectance, and fluorescence, are used to characterize chemical structure and photoluminescence of these polymers. The polyphenols exhibit fluorescence with significant stokes shift in the range 30-100 nm rendering them useful in fluorescence quenching-based sensors. Preliminary studies on use of these polymers, in the detection of nitro-aromatic compounds in solution through using fluorescence-quenching are also presented. (c) 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46496.
机译：利用用于合成荧光共轭聚合物（CP）的金属催化剂的公认的缺点是需要广泛纯化，以确保完全除去将终止荧光的残留催化剂。此外，荧光CP的典型合成涉及具有不同毒性水平的多个步骤，单体和溶剂。该作业证明了利用氧化还原酶作为催化剂的可能性，用于天然存在的酚类聚合以产生荧光共轭多酚。酶的活性位点中的金属在合成期间保持螯合，允许聚合物如合成的，而不需要大量纯化。使用辣根过氧化物酶作为生物催化剂聚合三种天然酚，4-羟基苯基乙酸，羟基吡啶醇和绿原酸。光谱技术，UV-Vis，傅里叶变换红外光谱衰减的总反射率和荧光用于表征这些聚合物的化学结构和光致发光。多酚在30-100nm的范围内显示出具有显着的斯托克的荧光，使其可用于基于荧光猝灭的传感器。还介绍了使用荧光淬火的溶液中硝基芳族化合物在检测中使用这些聚合物的初步研究。 （c）2018 Wiley期刊，Inc.J.Phill。聚合物。 SCI。 2018,135,46496。

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