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首页> 外文期刊>Journal of Cell Science >Constitutive IP(3)R1-mediated Ca2+ release reduces Ca2+ store content and stimulates mitochondrial metabolism in mouse GV oocytes
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Constitutive IP(3)R1-mediated Ca2+ release reduces Ca2+ store content and stimulates mitochondrial metabolism in mouse GV oocytes

机译：组成型IP（3）R1介导的CA2 +释放减少CA2 +储存含量，刺激小鼠GV卵母细胞中的线粒体代谢

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In mammals, fertilization initiates Ca2+ oscillations in metaphase II oocytes, which are required for the activation of embryo development. Germinal vesicle (GV) oocytes also display Ca2+ oscillations, although these unfold spontaneously in the absence of any known agonist(s) and their function remains unclear. We found that the main intracellular store of Ca2+ in GV oocytes, the endoplasmic reticulum ([Ca2+](ER)), constitutively 'leaks' Ca2+ through the type 1 inositol 1,4,5-trisphosphate receptor. The [Ca2+](ER) leak ceases around the resumption of meiosis, the GV breakdown (GVBD) stage, which coincides with the first noticeable accumulation of Ca2+ in the stores. It also concurs with downregulation of the Ca2+ influx and termination of the oscillations, which seemed underpinned by the inactivation of the putative plasma membrane Ca2+ channels. Lastly, we demonstrate that mitochondria take up Ca2+ during the Ca2+ oscillations, mounting their own oscillations that stimulate the mitochondrial redox state and increase the ATP levels of GV oocytes. These distinct features of Ca2+ homeostasis in GV oocytes are likely to underpin the acquisition of both maturation and developmental competence, as well as fulfill stage-specific cellular functions during oocyte maturation.
机译：在哺乳动物中，施肥引发了中期II卵母细胞中的Ca2 +振荡，这是激活胚胎发育所必需的。发芽囊泡（GV）卵母细胞也显示CA2 +振荡，尽管在不存在任何已知的激动剂的情况下自发地展开并且它们的功能仍然不清楚。我们发现Ca2 +在GV卵母细胞中的主要细胞内储存，内质网（[Ca2 +]（ER）），构成型'泄漏的Ca2 +通过1型肌醇1,4,5-三磷酸酯受体。 [CA2 +]（ER）泄漏停止恢复减数分裂，GV击穿（GVBD）阶段，它与商店中CA2 +的第一个显着积累一致。它还同时进行CA2 +流入和偏离振荡的下调，似乎通过推定的血浆膜CA2 +通道的失活效果是基础的。最后，我们证明了线粒体在CA2 +振荡期间占据了CA2 +，安装了自己的振荡，刺激线粒体氧化还原状态并增加GV卵母细胞的ATP水平。在GV卵母细胞中，CA2 +稳态的这些不同的特征可能是在卵母细胞成熟期间获得成熟和发育能力的收购，以及满足特异性阶段特异性细胞功能。

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