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首页> 外文期刊>Journal of Cell Science >Increasing ergosterol levels delays formin-dependent assembly of F-actin cables and disrupts division plane positioning in fission yeast
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Increasing ergosterol levels delays formin-dependent assembly of F-actin cables and disrupts division plane positioning in fission yeast

机译：增加Ergosterol水平延迟F-Actin电缆的依赖性组装，并破坏裂变酵母中的分裂平面定位

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In most eukaryotes, cytokinesis is mediated by the constriction of a contractile acto-myosin ring (CR), which promotes the ingression of the cleavage furrow. Many components of the CR interact with plasma membrane lipids suggesting that lipids may regulate CR assembly and function. Although there is clear evidence that phosphoinositides play an important role in cytokinesis, much less is known about the role of sterols in this process. Here, we studied how sterols influence division plane positioning and CR assembly in fission yeast. We show that increasing ergosterol levels in the plasma membrane blocks the assembly of F-actin cables from cytokinetic precursor nodes, preventing their compaction into a ring. Abnormal F-actin cables form after a delay, leading to randomly placed septa. Since the formin Cdc12 was detected on cytokinetic precursors and the phenotype can be partially rescued by inhibiting the Arp2/3 complex, which competes with formins for F-actin nucleation, we propose that ergosterol may inhibit formin dependent assembly of F-actin cables from cytokinetic precursors
机译：在大多数真核生物中，胞质分裂是由收缩雅图肌球蛋白环（CR），其促进卵裂沟的侵入的收缩介导的。与血浆膜脂质表明脂质可以组装和功能调节CR的CR相互作用的许多组件。尽管有明确的证据表明磷酸肌醇发挥胞质分裂的重要作用，更有人知道固醇在这个过程中的作用。在这里，我们研究如何固醇裂殖酵母影响分割平面定位与CR组装。我们表明，增加质膜块麦角甾醇水平的F-肌动蛋白的电缆组件从cytokinetic前体节点，防止其压实成环。异常F-肌动蛋白的延迟之后电缆形成，导致随机放置隔片。由于formin CDC12已于cytokinetic前体检测和表型可通过抑制的Arp2 /部分地救出3复合物，其与formins对竞速赛F-肌动蛋白成核，我们建议，麦角甾醇可能抑制formin的依赖组件F-肌动蛋白从cytokinetic电缆前体

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