...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cell Science >PLK4 is a microtubule-associated protein that self-assembles promoting de novo MTOC formation
【24h】

PLK4 is a microtubule-associated protein that self-assembles promoting de novo MTOC formation

机译：PLK4是一种微管相关蛋白，其自组装促进De Novo MTOC形成

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The centrosome is an important microtubule-organising centre (MTOC) in animal cells. It consists of two barrel-shaped structures, the centrioles, surrounded by the pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to an existing structure (canonical duplication) or de novo . How centrosomes form de novo is not known. The master driver of centrosome biogenesis, PLK4, is critical for the recruitment of several centriole components. Here, we investigate the beginning of centrosome biogenesis, taking advantage of Xenopus egg extracts, where PLK4 can induce de novo MTOC formation (Eckerdt et al., 2011; Zitouni et al., 2016). Surprisingly, we observe that in vitro , PLK4 can self-assemble into condensates that recruit α- and β-tubulins. In Xenopus extracts, PLK4 assemblies additionally recruit STIL, a substrate of PLK4, and the microtubule nucleator γ-tubulin, forming acentriolar MTOCs de novo . The assembly of these robust microtubule asters is independent of dynein, similar to what is found for centrosomes. We suggest a new mechanism of action for PLK4, where it forms a self-organising catalytic scaffold that recruits centriole components, PCM factors and α- and β-tubulins, leading to MTOC formation.This article has an associated First Person interview with the first author of the paper.
机译：Centrosome是动物细胞中的重要微管组织中心（MTOC）。它由两个筒形结构，半桶形结构组成，围绕蠕虫菊属材料（PCM）包围，其核解微管。 Centrosomes可以接近现有的结构（规范重复）或De Novo。 CentroSomes如何形成De Novo尚不清楚。 Centroosome生物生物的主驱动器PLK4对于募集几个厘级组件至关重要。在这里，我们调查了基础生物发生的开始，利用外爪卵蛋提取物，其中PLK4可以诱导De Novo MTOC形成（Eckerdt等，2011; Zitouni等，2016）。令人惊讶的是，我们观察到体外，PLK4可以自组装成募集α-和β-管蛋白的缩合物。在外爪疮提取物中，PLK4组件另外募集STIL，PLK4的基材和微管核酸γ-管蛋白，形成aceTRILAR MTOCS de Novo。这些强大的微管紫苑的组装与Dynein无关，类似于对中心的发现。我们为PLK4建议了一种新的行动机制，它形成了一种自组织催化支架，促进了离心组分，PCM因子和α-和β-管蛋白，导致MTOC地层。本文有一个相关的第一个人访谈第一个本文的作者。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号