From batch to continuous chromatographic purification of a therapeutic peptide through multicolumn countercurrent solvent gradient purification

De Luca Chiara; Felletti Simona; Lievore Giulio; Buratti Alessandro; Vogg Sebastian; Morbidelli Massimo; Cavazzini Alberto; Catani Martina; Macis Marco; Ricci Antonio; Cabri Walter

首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >From batch to continuous chromatographic purification of a therapeutic peptide through multicolumn countercurrent solvent gradient purification

【24h】

From batch to continuous chromatographic purification of a therapeutic peptide through multicolumn countercurrent solvent gradient purification

机译：通过批量来通过多穴逆流溶剂梯度纯化来连续色谱纯化治疗肽

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A twin-column Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) process has been developed for the purification of a therapeutic peptide, glucagon, from a crude synthetic mixture. This semicontinuous process uses two identical columns operating either in interconnected or in batch mode, thus enabling the internal recycle of the portions of the eluting stream which do not comply with purity specifications. Because of this feature, which actually results in the simulated countercurrent movement of the stationary phase with respect to the mobile one, the yield-purity trade-off typical of traditional batch preparative chromatography can be alleviated. Moreover, the purification process can be completely automatized.

机译：已经开发了双柱多柱逆流溶剂梯度纯化（MCSGP）工艺用于从粗合成混合物中纯化治疗肽胰高血糖素。该半连续过程使用两种相互作用的柱，其在互连或批量模式下操作，从而使洗脱流的部分的内部再循环，其不符合纯度规格。由于该特征，其实际上导致静止相对于移动相位的模拟逆流运动，可以缓解传统分批制备色谱的典型的产量纯度折衷。此外，净化过程可以完全自动化。

著录项

来源
《Journal of chromatography, A: Including electrophoresis and other separation methods》 |2020年第1期|共7页
作者
De Luca Chiara; Felletti Simona; Lievore Giulio; Buratti Alessandro; Vogg Sebastian; Morbidelli Massimo; Cavazzini Alberto; Catani Martina; Macis Marco; Ricci Antonio; Cabri Walter;
展开▼
作者单位

Univ Ferrara Dept Chem &

Pharmaceut Sci Via L Borsari 46 I-44121 Ferrara Italy;

Univ Ferrara Dept Chem &

Pharmaceut Sci Via L Borsari 46 I-44121 Ferrara Italy;

Univ Ferrara Dept Chem &

Pharmaceut Sci Via L Borsari 46 I-44121 Ferrara Italy;

Univ Ferrara Dept Chem &

Pharmaceut Sci Via L Borsari 46 I-44121 Ferrara Italy;

Swiss Fed Inst Technol Inst Chem &

Bioengn Vladimir Prelog Weg 1 CH-8093 Zurich Switzerland;

Swiss Fed Inst Technol Inst Chem &

Bioengn Vladimir Prelog Weg 1 CH-8093 Zurich Switzerland;

Univ Ferrara Dept Chem &

Pharmaceut Sci Via L Borsari 46 I-44121 Ferrara Italy;

Univ Ferrara Dept Chem &

Pharmaceut Sci Via L Borsari 46 I-44121 Ferrara Italy;

Fresenius Kabi iPSUM Via San Leonardo 23 I-45010 Rovigo Italy;

Fresenius Kabi iPSUM Via San Leonardo 23 I-45010 Rovigo Italy;

Fresenius Kabi iPSUM Via San Leonardo 23 I-45010 Rovigo Italy;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类分析化学;
关键词
Preparative chromatography; Continuous chromatography; Peptide purification; Multicolumn countercurrent solvent gradient process;

机译：制备色谱;连续色谱;肽纯化;多块逆流溶剂梯度过程;

相似文献

外文文献
中文文献
专利

1. From batch to continuous chromatographic purification of a therapeutic peptide through multicolumn countercurrent solvent gradient purification [J] . De Luca Chiara, Felletti Simona, Lievore Giulio, Journal of chromatography, A: Including electrophoresis and other separation methods . 2020,第1期

机译：通过批量来通过多穴逆流溶剂梯度纯化来连续色谱纯化治疗肽
2. Chromatographic separation of three monoclonal antibody variants using multicolumn countercurrent solvent gradient purification (MCSGP) [J] . Muller-Spath T, Aumann L, Melter L, Biotechnology and Bioengineering . 2008,第6期

机译：使用多柱逆流溶剂梯度纯化（MCSGP）色谱分离三种单克隆抗体变体
3. A continuous multicolumn countercurrent solvent gradient purification (MCSGP) process [J] . Aumann L, Morbidelli M Biotechnology and Bioengineering . 2007,第5期

机译：连续多柱逆流溶剂梯度纯化（MCSGP）工艺
4. Continuous Countercurrent Tangential Chromatography For Purification of High Value Therapeutic Proteins [C] . Andrew Zydney, Oleg Shinkash, Boris Napadensky, Integrated continuous biomanufacturing II . 2015

机译：连续逆流切向色谱法纯化高价值的治疗性蛋白质
5. Gradient chromatofocusing: A new chromatographic technique for protein purification and separation. [D] . Shan, Lian. 2001

机译：梯度色谱聚焦：一种用于蛋白质纯化和分离的新色谱技术。
6. Partition Efficiency of High-Pitch Locular Multilayer Coil for Countercurrent Chromatographic Separation of Proteins Using Small-Scale Cross-Axis Coil Planet Centrifuge and Application to Purification of Various Collagenases with Aqueous-Aqueous Polymer Phase Systems [O] . Kazufusa Shinomiya, Hiroko Kobayashi, Norio Inokuchi, -1

机译：用小尺寸横轴线圈线离心机离心蛋白质逆流色谱分离蛋白质的逆流色谱分离的分配效率并用含水聚合物相位系统纯化各种胶原蛋白酶的纯化
7. Continuous countercurrent chromatographic separator for the purification of sugars from biomass hydrolyzate. Final project report, July 1, 1996--September 30, 1997 [O] . Wooley, R. J. 1997

机译：连续逆流色谱分离器，用于从生物质水解产物中纯化糖。最终项目报告，1996年7月1日 - 1997年9月30日
8. Continuous countercurrent chromatographic separator for the purification of sugars from biomass hydrolyzate. Final project report, July 1, 1996--September 30, 1997 [R] . Wooley, R. J. 1997

机译：连续逆流色谱分离器，用于从生物质水解产物中纯化糖。最终项目报告，1996年7月1日 - 1997年9月30日

From batch to continuous chromatographic purification of a therapeutic peptide through multicolumn countercurrent solvent gradient purification

摘要

著录项

相似文献

相关主题

期刊订阅