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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Effect of buffer nature and concentration on the chromatographic performance of basic compounds in the absence and presence of 1-hexyl-3-methylimidazolium chloride
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Effect of buffer nature and concentration on the chromatographic performance of basic compounds in the absence and presence of 1-hexyl-3-methylimidazolium chloride

机译：缓冲性质和浓度对碱性化合物的不存在和存在的碱性化合物的色谱性能的影响及存在的1-己基-3-甲基咪唑氯化物

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In reversed-phase liquid chromatography, the performance for basic compounds is affected by the interaction of the protonated (cationic) species with the anionic free silanols on the alkyl-bonded stationary phases. Using aqueous-organic mobile phases in the absence of additives, the retention may be too high, and the peaks be broad and asymmetric. The performance is improved by addition to the mobile phase of ionic liquids, from which 1-hexyl-3-methylimidazolium chloride ([C(6)MIm][CI]) has especially good characteristics. A recent report has also revealed that the use of the phosphate system as buffer, at varying concentration and pH, may have a significant role in the chromatographic performance of basic compounds, with effects on both retention and peak shape. In this work, this study has been extended to other three buffer systems (acetate, citrate, and formate), at increasing concentrations and pH 3 and 7, in the presence and absence of [C(6)MIm][CI]. The results have been compared with those obtained with the phosphate system. The retention increases by addition of larger concentration of all buffers, in both absence and presence of [C(6)MIm][CI]. Without additive, peak performance is also enhanced significantly. This effect is minimal in the presence of [C(6)MIm][CI], which yields highly symmetrical peaks at all buffer concentrations, due to an effective blocking of the silanol activity. (C) 2019 Elsevier B.V. All rights reserved.
机译：在反相液相色谱中，基本化合物的性能受质子化（阳离子）物种与阴离子游离硅烷醇对烷基键合的固定相的相互作用的影响。在没有添加剂的情况下使用水性有机流动阶段，保持率太高，峰值宽而不对称。除了离子液体的流动相的外，可以改善性能，从中，从中，1-己基-3-甲基咪唑鎓氯化物（[C（6）mim] [Ci]）具有特别好的特性。最近的报告还揭示了使用磷酸盐体系作为缓冲液，以不同的浓度和pH，在碱性化合物的色谱性能中可能具有重要作用，具有对保留和峰形的影响。在这项工作中，该研究已经扩展到其他三种缓冲系统（醋酸盐，柠檬酸盐和甲酸），在增加和不存在[C（6）MIM] [CI]的情况下，浓度和pH 3和7。将结果与用磷酸盐体系获得的结果进行了比较。通过加入较大所有缓冲液的浓度的滞留增加，在两个不存在和[C（6）MIM] [Cl]的存在下进行。没有添加剂，峰值性能也显着增强。在[C（6）MIM] [CI]存在下，这种效果最小，由于硅烷醇活性的有效阻断，在所有缓冲浓度下产生高度对称的峰。 （c）2019 Elsevier B.v.保留所有权利。

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