...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry detection with a 50 ms modulation period
【24h】

Comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry detection with a 50 ms modulation period

机译：综合二维气相色谱和飞行时间质谱检测，50 ms调制周期

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An ultrafast flow modulation period, P-M of 50 ms, for comprehensive two-dimensional (2D) gas chromatography (GC x GC) with time-of-flight mass spectrometry (TOFMS) detection is demonstrated, producing narrow peak widths, W-2(4 sigma width-at-base on the D-2 dimension), demonstrating the potential for ultrafast (2D) separations with high peak capacity. The modulator is a pulse flow valve that injects a narrow pulse of carrier gas at a user defined P-M, at the union between the D-1 and D-2 columns. The raw data produced combines the properties of vacancy chromatography and frontal analysis. Deconvolution of the raw data using unconstrained multivariate curve resolution alternating least squares (MCR-ALS) analysis facilitates identification and quantification for overlapped analyte peaks. The peak profile loadings obtained from MCR-ALS are converted into traditional appearing GC x GC data through a process commonly used with frontal analysis. An 18-component test mixture at seven different injected mass levels was studied. The D-2 peaks generated ranged from an W-2 of 16 to 36 ms with an average of 26 ms. At an on-column injected mass of 14 ng per analyte, an average mass spectral match value, MV, of 822 was achieved using in-house collected spectra for comparison, with an average match value RSD of 7.1%. Calibration of overlapped test analytes was evaluated using the areas of the MCR-ALS loadings, with excellent quantification demonstrated. The advancement demonstrated in modulation performance for GC x GC represents a significant decrease in P-M as most commercial modulators have a minimum P-M of 1 s, while maintaining the benefits of a duty cycle of essentially 1.0, which promises to enable new chemical analyzer designs, compatible with the vacuum requirements of the TOFMS detector. (C) 2018 Elsevier B.V. All rights reserved.
机译：对综合二维（2D）气相色谱（GC X GC）具有飞行时间质谱（TOFMS）检测的超快流量调制周期，PM为50ms，产生窄峰宽度，W-2（ D-2尺寸的4个Sigma宽度底座），展示超快（2D）具有高峰容量的电位。调制器是脉冲流量阀，其在D-1和D-2列之间的UNION处在用户定义的P-M处注入载气的窄脉冲。产生的原始数据结合了空位色谱和额度分析的性质。使用无约束多变量曲线分辨率交替最小二乘（MCR-ALS）分析的原始数据的去卷积有助于识别和定量重叠的分析物峰。通过常规分析通常使用的方法将从MCR-ALS获得的峰值轮廓加载到传统的GC X GC数据中。研究了七种不同注入质量水平的18组分试验混合物。产生的D-2峰值范围为16至36ms的W-2，平均为26ms。在每分析物的14ng的柱状物质中，使用内部收集的光谱实现了822的平均质谱匹配值MV，以进行比较，平均匹配值RSD为7.1％。使用MCR-ALS载荷的区域评估重叠测试分析物的校准，并证明了优异的量化。在GC X GC的调制性能下表现出的进步表示PM的显着降低，因为大多数商业调制器具有最小PM的PM为1 S，同时保持基本上1.0的占空比的好处，这承诺使新的化学分析仪设计能够设计，兼容具有TOFMS检测器的真空要求。 （c）2018年elestvier b.v.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号