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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Preparation of titanium ion functionalized polydopamine coated ferroferric oxide core-shell magnetic particles for selective extraction of nucleotides from Cordyceps and Lentinus edodes
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Preparation of titanium ion functionalized polydopamine coated ferroferric oxide core-shell magnetic particles for selective extraction of nucleotides from Cordyceps and Lentinus edodes

机译：钛离子官能化聚二胺涂覆铁铁氧化物核心 - 壳磁性颗粒的制备，用于选择性提取冬虫夏草和Lentinus edodes的核苷酸

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摘要

In this study, a titanium ion (Ti4+) functionalized polydopamine coated ferroferric oxide (Fe3O4@PDA@Ti4+) core-shell magnetic particle was prepared for the selective extraction of nucleotides. Firstly, different metal ions including Ti4+, Zr4+, Fe3+, Al3+, Cu2+, Zn2+, Ni2+ and Mg2+ were respectively immobilized onto Fe3O4@PDA particles and their extraction efficiency for five nucleotides [cytidine-5'-monophosphate (CMP), uridine-5'-monophosphate (UMP), guanosine-5'-monophosphate (GMP), thymidine-5'-monophosphate (TMP) and adenosine-5'-monophosphate (AMP)] were compared. Among these prepared materials, Fe3O4@PDA@Ti4+, which exhibited the highest extraction efficiency for nucleotides, was further characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy. After being optimized of the extraction parameters including adsorbent amounts, extraction time, extraction temperature, type and concentration of the eluent, the prepared Fe3O4@PDA@Ti4+ magnetic particles were successfully applied for the selective extraction and determination of CMP, UMP, GMP, TMP and AMP in Cordyceps and Lentinus edodes. Good linearity (varying from 0.063 to 19.000 mu g/mL, R-2 > 0.999) and low limit of detection (LODs) (ranging between 0.0047 and 0.0141 mu g/mL) for target analytes were achieved. These results demonstrated that the synthesized material in this study had potential for selective extraction of phosphorylated small molecular compounds in complicated matrix. (C) 2019 Elsevier B.V. All rights reserved.
机译：在该研究中，制备钛离子（Ti4 +）官能化的聚德莫胺涂覆的铁氧化物（Fe3O4 @ Ti4 +）核 - 壳磁性颗粒用于选择性提取核苷酸。首先，将包括Ti4 +，Zr4 +，Fe 3 +，Al 3 +，Cu 2 +，Zn2 +，Ni2 +和Mg2 +的不同金属离子分别将其固定在Fe 3 O 4颗粒上并进行5个核苷酸的提取效率[胞嘧啶-5'-单磷酸（CMP），尿苷-5比较了“ - 磷酸（UMP），鸟苷-5'-单磷酸（GMP），胸苷-5'-单磷酸（TMP）和腺苷-5'-单磷酸（AMP）。在这些制备的材料中，通过傅里叶变换红外光谱，扫描电子显微镜，透射电子显微镜和能量分散X射线光谱，进一步表征了核苷酸最高提取效率的Fe3O4 @ PDA @ Ti4 +。优化包括吸附量的提取参数，提取时间，提取温度，型洗脱液的型型和浓度，制备的Fe3O4 @ PDA-Ti4 +磁性颗粒已成功应用于CMP，UMP，GMP，TMP的选择性提取和测定和冬虫夏草和戊糖豆的amp。实现了良好的线性（从0.063至19.000μmg/ ml，R-2> 0.999）和低检测限（LOD）（0.0047和0.0041μg/ ml）的低限制，得到靶分析物。这些结果表明，该研究的合成材料具有选择性提取复杂基质中磷酸化的小分子化合物的可能性。 （c）2019 Elsevier B.v.保留所有权利。

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