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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Development of a rapid ultra performance hydrophilic interaction liquid chromatography tandem mass spectrometry method for procyanidins with enhanced ionization efficiency
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Development of a rapid ultra performance hydrophilic interaction liquid chromatography tandem mass spectrometry method for procyanidins with enhanced ionization efficiency

机译：一种快速超高性能亲水性相互作用液相色谱串联质谱法，采用增强电离效率

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Cocoa flavanols (catechins and procyanidins) can exist in various polymerization states and are commonly classified by their degree of polymerization (DP). There is increasing evidence that flavanols of distinct DP possess different biological activities, but separation and quantification of the higher DP procyanidins is challenging and has thus created the need for new methodologies that utilize advancements in columns and LC-MS/MS systems. An aqueous normal phase (hydrophilic interaction liquid chromatography, HILIC), UPLC method with post-column ESI adjuvant infusion was developed to reduce the total analysis time, increase peak separation, and increase detection specificity (compared to traditional fluorescence methods) by coupling with mass spectrometry detection. The total elution time was reduced from 70 to 90 min (typically used for normal phase and HILIC HPLC separation of procyanidins) down to 9 min by employing UPLC. Results indicate that by using a post-column 0.04 M ammonium formate infusion (5 mu L/min), ionization of procyanidins was significantly enhanced. Lower limits of detection ranged from 3.19 x 10(-2) to 4.56 pmol-on-column, and lower limits of quantification ranged from 2.79 x 10(-2) to 1.17 x 10(2) pmol-on-column across compounds DP 1-9. This method builds upon the foundation set by existing analytical methods and employs new technologies to dramatically increase sample throughput and enhance detection limits and specificity, facilitating improved analysis for procyanidins. (C) 2019 Elsevier B.V. All rights reserved.
机译：可以在各种聚合状态中存在可可氟烷醇（儿茶素和草原），并且通常通过它们的聚合度（DP）分类。越来越多的证据表明，不同DP的黄黄醇具有不同的生物学活性，但较高的DP ProCyanidins的分离和定量是具有挑战性的，因此因此创造了利用列和LC-MS / MS系统的进步的新方法。具有柱子后ESI佐剂输注的水性正常相（亲水性相互作用液相色谱，HILIC），通过与质量耦合来减少总分析时间，增加峰分离，增加检测特异性（与传统荧光方法相比）增加光谱检测。通过采用UPLC，总洗脱时间从70〜90分钟（通常用于正常相和正常相和HILIC HPLC分离）下降至9分钟。结果表明，通过使用柱后0.04M甲酸氨酸输注（5μl/ min），显着提高了原花青素的电离。较低的检测限范率为3.19×10（-2）至4.56 pmol柱，横跨化合物DP的2.79×10（2）pMol-on-thop的较低的定量限制1-9。该方法在现有分析方法的基础上建立了基础，采用新技术，从而大大提高样品吞吐量，增强检测限和特异性，促进对原因糖苷的改进分析。 （c）2019 Elsevier B.v.保留所有权利。

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