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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Large-scale separation of baicalin and wogonoside from Scutellaria Baicalensis Georgi by the combination of pH-zone-refining and conventional counter-current chromatography
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Large-scale separation of baicalin and wogonoside from Scutellaria Baicalensis Georgi by the combination of pH-zone-refining and conventional counter-current chromatography

机译：通过pH区炼油和常规反电流色谱法从Scutellaria baicalensis Georgi的大规模分离瘢痕疙瘩和Wogonoside

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摘要

The combined use of pH-zone-refining counter-current chromatography (CCC) and conventional CCC was successfully employed for the preparative separation and purification of baicalin and wogonoside from the traditional Chinese medicinal herb Scutellaria Baicalensis Georgi. A more environmentally friendly biphasic solvent system composed of n-butanol-ethyl acetate-water (2:3:5, viv) was used for pH-zone refining CCC, in which trifluoroacetic acid was added to a final concentration of 10 mmol L-1 in the organic phase and ammonia was added to a final concentration of 10 mmol L-1 in the aqueous phase while a two-phase solvent system composed of ethyl acetate-ethanol-3 mmol L-1 hydrochloric acid (10:1:10, v/v) was used for the conventional CCC. Two elution modes including reverse displacement mode and normal displacement mode were investigated for pH-zone-refining CCC. The first run provided 186.7 mg of baicalin with a purity of 95.3% and 143.4 mg of a mixture of baicalin and wogonoside from 500 mg of the crude extracts of Scutellaria Baicalensis Georgi by pH-zone-refining CCC with reverse displacement elution mode. The mixture was further separated by means of a second run in conventional CCC, yielding 64.3 mg of baicalin with a purity of 98.2% and 46.1 mg of wogonoside with a purity of 98.9%. (C) 2019 Elsevier B.V. All rights reserved.
机译：组合使用pH区带精制逆流色谱（CCC）和常规的CCC被成功地用于从传统的中国草药黄芩黄芩苷和汉黄芩苷的制备分离和纯化。正丁醇 - 乙酸乙酯 - 水组成的更加环境友好的两相溶剂体系（2：3：5，VIV）用于pH区带精制CCC，其中三氟乙酸加至10毫摩尔的最终浓度L- 1在有机相中和氨加入到10毫摩尔L-1的在水相中的最终浓度而两相溶剂系统组成的乙酸乙酯 - 乙醇 - 3毫摩尔L-1的盐酸（10：1：10 ，v / v）用于常规CCC。两种洗脱模式包括反向位移模式和正常排量模式进行了调查的pH区域精制CCC。在第一次运行提供186.7毫克黄芩苷具有95.3％的纯度和143.4毫克通过用反向位移洗脱模式的pH区域精制CCC一个黄芩甙，从500毫克黄芩的粗提取物的汉黄芩苷的混合物组成。将该混合物通过在常规CCC第二次运行的装置进一步分离，得到64.3毫克黄芩苷98.2％的纯度，并用98.9％的纯度46.1毫克汉黄芩苷的。 （c）2019 Elsevier B.v.保留所有权利。

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