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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Oligosaccharide-marker approach for qualitative and quantitative analysis of specific polysaccharide in herb formula by ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry: Dendrobium officinale, a case study
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Oligosaccharide-marker approach for qualitative and quantitative analysis of specific polysaccharide in herb formula by ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry: Dendrobium officinale, a case study

机译：通过超高效液相色谱 - 四桥 - 飞行时间质谱法测定特异性多糖的定性和定量分析特异性多糖的定性和定量分析的寡核糖 - 标记分析方法：Dendrobium Officinale，案例研究

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摘要

Qualitative and quantitative analysis of polysaccharides in herb formula remain challenge due to the limited choices of analytical methods concerning the intrinsic characteristics of large molecular mass. Herein, an oligosaccharide-marker approach was newly developed for quality assessment of polysaccharides in herbal materials, using Dendrobium officinale as a case study. This method involved partial acid hydrolysis of D. officinale polysaccharide (DOP) followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Two ABEE-labeled oligosaccharides namely, Te-Man-ABEE and Pen-Man-ABEE, were selected as chemical markers due to their high specificity in herb formula. The linear relationship between the content of these two markers and the content of DOP was then successfully established respectively. The linear relationship was further transformed to that between peak area of chemical markers and DOP content so that chemical markers were not necessary to be isolated for analysis. This linear relationship was systemically validated in terms of precision and accuracy. The results showed that these two oligosaccharide-markers presented a good linear relationship with DOP (R-2 >= 0.997) in the range of 0.68-16.02 mu g. These markers also demonstrated satisfactory precision (RSD < 7.0%), and recovery (91.41%-118.30%) in real sample determination. Additionally, there was no significant difference between the results given by the two chemical markers as the RSD values were not more than 7.0%. While concerning the results given by the oligosaccharide-markers and the previously-published polysaccharide marker, the RSD value was not more than 6.4%. These suggest that the oligosaccharide-marker approach is a simple, quick, and reliable method to qualitatively and quantitatively determine of specific polysaccharide in herb formula. (C) 2019 Elsevier B.V. All rights reserved.
机译：由于大分子量的内在特征的分析方法的有限选择，Herb公式的多糖定性和定量分析仍然挑战。在此，使用Dendrobium Officinale作为案例研究，新开发了寡糖 - 标记方法以进行多糖质量评估。该方法涉及D. officinale多糖（DOP）的部分酸水解，然后是对氨基苯甲酸乙酯（ABEE）衍生化。由于它们在草本公式中的高特异性，选择了两种Abee标记的寡糖，即Te-Man-Abee和Pen-Man-abee。然后成功建立了这两个标记的含量与DOP含量之间的线性关系。进一步转化为线性关系，在化学标志物和DOP含量的峰面积之间，使得不需要分离化学标记物进行分析。这种线性关系在精度和准确性方面全身验证。结果表明，这两种寡糖标记物与DOP（R-2> = 0.997）呈现出良好的线性关系，范围为0.68-16.02μg。这些标志物还证明了令人满意的精度（RSD <7.0％），并在实际样品测定中恢复（91.41％-118.30％）。另外，由于RSD值不超过7.0％，两种化学标记给出的结果没有显着差异。虽然关于寡糖标记物和先前公布的多糖标记给出的结果，但RSD值不大于6.4％。这些表明，寡糖标记方法是一种简单，快速，可靠的方法，可定义和定量地确定草药中的特异性多糖。 （c）2019 Elsevier B.v.保留所有权利。

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