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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >A comprehensive study of pomegranate flowers polyphenols and metabolites in rat biological samples by high-performance liquid chromatography quadrupole time-of-flight mass spectrometry
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A comprehensive study of pomegranate flowers polyphenols and metabolites in rat biological samples by high-performance liquid chromatography quadrupole time-of-flight mass spectrometry

机译：高效液相色谱运动液相色谱综合研究大鼠生物样品中石榴花多酚和代谢物的综合研究

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Pomegranate flowers is an ancient medicine that has commonly been used to treat various diseases such as diabetes. However, no reports are available on the metabolic profile of pomegranate flowers in vivo. In the present study, with the aid of HPLC-Q-TOF-MS2, 67 compounds were identified in pomegranate flowers extract, including 18 ellagitannins, 14 gallic acid and galloyl derivatives, five anthocyanins and 18 flavonoids. Seven compounds were firstly identified. In vivo, 22 absorbed compounds and 35 metabolites were identified in rat biosamples (urine, feces, plasma and tissues) after orally administered with pomegranate flowers extract. This result showed that not all compounds abundant in pomegranate flowers extract could be absorbed well in plasma and tissues. This finding also suggested a potential correlation between study on metabolic profile of these compounds in vivo and study on strategy of screening bioactivity of the isolates with in vitro cell systems evaluation. Notably, mono-glucuronide conjugated metabolite of ellagitannin compound (corilagin) was firstly identified. In addition, this is first report to identify phase II conjugate metabolites of ellagitannins in vivo after oral administration of ellagitanninsrich extracts (or foods). Thus, characterizing its multiple constitution, absorption and metabolic fate of these compounds in vivo is helpful to better analyze the active components in pomegranate flowers. (C) 2019 The Authors. Published by Elsevier B.V.
机译：石榴花是一种古老的药物，通常用于治疗糖尿病等各种疾病。但是，没有报告在体内石榴花的代谢剖面上提供。在本研究中，借助HPLC-Q-TOF-MS2，在石榴花提取物中鉴定了67种化合物，其中包括18个壳蛋白酶，14个无碱酸和Galloyl衍生物，五种花青素和18种黄酮。首先鉴定出七种化合物。在口服用石榴花提取物口服施用之后，在大鼠生物素（尿液，粪便，血浆和组织中，鉴定了22种吸收的化合物和35种代谢物。该结果表明，在血浆和组织中，不是石榴花提取物中的所有化合物都可以良好吸收。该发现还建议研究这些化合物在体内这些化合物的代谢概况与筛选与体外细胞体系评价的分离株策略的研究潜在的相关性。值得注意的是，首先鉴定出素葡萄糖蛋白化合物（Corilagin）的单葡糖醛酸缀合代谢物。此外，这是首先报告鉴定在口服埃尔拉根anchinsrich提取物（或食物）后体内体内鞣菌蛋白酶缀合物的蛋白质代谢物。因此，表征其在体内中这些化合物的多种构成，吸收和代谢命运有助于更好地分析石榴花中的活性成分。 （c）2019年作者。由elsevier b.v出版。

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