Preparation of flavone di-C-glycoside isomers from Jian-Gu injection (Premna fulva Craib.) using recycling counter-current chromatography

Chen Yueyuan; Yan Xiaojie; Lu Fenglai; Jiang Xiaohua; Friesen J. Brent; Pauli Guido F.; Chen Shao-Nong; Li Dian-Peng

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Preparation of flavone di-C-glycoside isomers from Jian-Gu injection (Premna fulva Craib.) using recycling counter-current chromatography

机译：使用回收逆流色谱法制备Jian-Gu注射（Premna Fulva Craib）的黄酮二碳酸异构体

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Jian-Gu Injection (JGI, Premna fulva Craib) has been demonstrated to be effective in the clinical treatment of bone hyperplasia. However, an effective purification method of the JGI flavone C-glycosides as reference materials is not available. The present work developed a recycling counter-current chromatography approach to prepare these materials in high quality. An optimized biphasic solvent system composed of ethyl acetate/n-butanol/water (1:9:10, v/v/v) was employed to purify the five congeneric flavone C-glycosides, identified as apigenin 6,8-di-C-beta-D-glucopyranoside (vicenin 2), apigenin 6-C-beta-D-xylopyranosyl-8-C-beta-D-glucopyranoside (vicenin 1), apigenin 6-C-beta-n-xylopyranosyl-8-C-beta-D-galactopyranoside, apigenin 6-C-beta-D-glucopyranosyl-8-C-beta-D-xylopyranoside (vicenin 3), and apigenin 6-C-beta-D-galactopyranosyl-8-C-beta-D-xylopyranoside, by means of UHPLC-PDA-ESI-IT-ToF-MSn and NMR spectroscopy. (C) 2019 Elsevier B.V. All rights reserved.

机译：建2宫注射（JGI，Premna Fulva Craib）已被证明在骨质增生的临床治疗中是有效的。然而，不可用JGI黄酮C-甘油酯的有效纯化方法作为参考材料。本工作开发了一种回收逆流色谱法，以高质量的制备这些材料。使用由乙酸乙酯/正丁醇/水（1：9：10，v / v）组成的优化的双相溶剂体系，纯化五种基因的黄酮C-糖苷，鉴定为Apigenin 6,8-Di-C. -beta-d-吡喃吡喃糖苷（ViCenin 2），Apigenin 6-C-Beta-D- Xyloxylyl-8-C-β-D-吡喃葡萄糖苷（ViCenin 1），Apigenin 6-C-Beta-N- Xylopyranylyl-8-C. -beta-d-半乳糖醇，Apigenin 6-c-beta-d-吡喃葡萄糖基-8-c-beta-d-木嘧啶（vicenin 3）和亚替纳6-c-beta-d-半乳糖糖基-8-c-beta- 借助于UHPLC-PDA-ESI-TOF-MSN和NMR光谱，D-氧化吡喃糖苷。（c）2019 Elsevier B.v.保留所有权利。

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来源
《Journal of chromatography, A: Including electrophoresis and other separation methods》 |2019年第2019期|共7页
作者
Chen Yueyuan; Yan Xiaojie; Lu Fenglai; Jiang Xiaohua; Friesen J. Brent; Pauli Guido F.; Chen Shao-Nong; Li Dian-Peng;
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作者单位

Inst Bot Guangxi Key Lab Funct Phytochem Res &

Utilizat Guilin 541006 Guangxi Zhuang Peoples R China;

Inst Bot Guangxi Key Lab Funct Phytochem Res &

Utilizat Guilin 541006 Guangxi Zhuang Peoples R China;

Inst Bot Guangxi Key Lab Funct Phytochem Res &

Utilizat Guilin 541006 Guangxi Zhuang Peoples R China;

Inst Bot Guangxi Key Lab Funct Phytochem Res &

Utilizat Guilin 541006 Guangxi Zhuang Peoples R China;

Dominican Univ Rosary Coll Arts &

Sci Dept Phys Sci River Forest IL 60305 USA;

Inst Bot Guangxi Key Lab Funct Phytochem Res &

Utilizat Guilin 541006 Guangxi Zhuang Peoples R China;

Inst Bot Guangxi Key Lab Funct Phytochem Res &

Utilizat Guilin 541006 Guangxi Zhuang Peoples R China;

Inst Bot Guangxi Key Lab Funct Phytochem Res &

Utilizat Guilin 541006 Guangxi Zhuang Peoples R China;

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原文格式 PDF
正文语种 eng
中图分类分析化学;
关键词
Counter-current chromatography; Counter-current separation; Flavone C-glycosides; Premna fulva Craib; Jian-Gu injection;

机译：反电流色谱;反电流分离;黄酮C-糖苷;premna fulva craib;jian-gu journall;

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1. Preparation of flavone di-C-glycoside isomers from Jian-Gu injection (Premna fulva Craib.) using recycling counter-current chromatography [J] . Chen Yueyuan, Yan Xiaojie, Lu Fenglai, Journal of chromatography, A: Including electrophoresis and other separation methods . 2019,第期

机译：使用回收逆流色谱法制备Jian-Gu注射（Premna Fulva Craib）的黄酮二碳酸异构体
2. Separation of six compounds including two n-butyrophenone isomers and two stibene isomers from Rheum tanguticum Maxim by recycling high speed counter-current chromatography and preparative high-performance liquid chromatography [J] . Chen Tao, Yang Xue, Wang Nana, Journal of separation science. . 2018,第19期

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3. Bioassay-guided preparative separation of angiotensin-converting enzyme inhibitory C-flavone glycosides from Desmodium styracifolium by recycling complexation high-speed counter-current chromatography [J] . Zhang Ying-Qi, Luo Jian-Guang, Han Chao, Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis . 2015,第Null期

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机译：高速逆流色谱 - HPLC - DAD方法，用于从蒲公英宫内节育脉的两种聚甲氧化黄酮的制备分离和纯化

Preparation of flavone di-C-glycoside isomers from Jian-Gu injection (Premna fulva Craib.) using recycling counter-current chromatography

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