Directed evolution: the 'rational' basis for 'irrational' design

Tobin MB.; Huisman GW.; Gustafsson C.

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Directed evolution: the 'rational' basis for 'irrational' design

机译：定向进化：“非理性”设计的“理性”基础

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The development of powerful genetic manipulation formats has revolutionized the creation of functional biological molecules. Recent advances in directed evolution demonstrate that multiple properties of proteins can be optimized simultaneously and rapidly. Improved proteins often contain multiple and dispersed substitutions that act synergistically to improve enzyme properties and function. The benefits of such multiple changes are often not predictable from a priori structural knowledge. Furthermore, alternative solutions to gaining functional change can be obtained. [References: 57]

机译：强大的基因操纵形式的发展彻底改变了功能性生物分子的产生。定向进化的最新进展表明，可以同时快速地优化蛋白质的多种特性。改良的蛋白质通常包含多个分散的取代基，这些取代基具有协同作用，以改善酶的性质和功能。从先验的结构知识往往无法预测这种多重变化的好处。此外，可以获得获得功能更改的替代解决方案。 [参考：57]

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《Current Opinion in Structural Biology》 |2000年第4期|共7页
作者
Tobin MB.; Huisman GW.; Gustafsson C.;
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正文语种 eng
中图分类生物科学;
关键词
In-vitro evolution; Molecular evolution; Incremental truncation; Random mutagenesis; Protein evolution; Enzymes; Family; Biocatalysts; Esterase; Gene;

机译：体外进化;分子进化;截短;随机诱变;蛋白质进化;酶;家族;生物催化剂;酯酶;基因;

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1. Directed evolution: the 'rational' basis for 'irrational' design [J] . Tobin MB., Huisman GW., Gustafsson C. Current Opinion in Structural Biology . 2000,第4期

机译：定向进化：“非理性”设计的“理性”基础
2. Directed evolution relieves product inhibition and confers in vivo function to a rationally designed tyrosine aminotransferase. [J] . Rothman SC, Voorhies M, Kirsch JF Protein Science: A Publication of the Protein Society . 2004,第3期

机译：定向进化减轻了产物抑制，并使体内功能赋予了合理设计的酪氨酸转氨酶。
3. Bacterial cytochrome P450-catalyzed regio- and stereoselective steroid hydroxylation enabled by directed evolution and rational design [J] . Xiaodong Zhang, Yaqin Peng, Jing Zhao, Bioresources and Bioprocessing . 2020,第3期

机译：细菌细胞色素P450催化的regio-and立体选择性类固醇羟基化，通过定向演化和理性设计实现
4. Irrational-Dilation Orthonormal Wavelet Basis and its Calculation Method [C] . Hiroshi Toda, Zhong Zhang International Conference on Wavelet Analysis and Pattern Recognition . 2018

机译：无理扩张正交小波基及其计算方法
5. Rational design and directed evolution of novel streptavidin and intein mutants for biotechnology applications. [D] . DeMonte, Daniel William. 2015

机译：用于生物技术应用的新型链霉亲和素和内含肽突变体的合理设计和定向进化。
6. Directed evolution relieves product inhibition and confers in vivo function to a rationally designed tyrosine aminotransferase [O] . Steven C. Rothman, Mark Voorhies, Jack F. Kirsch 2004

机译：定向进化消除了产物抑制并赋予了体内设计合理的酪氨酸转氨酶功能
7. Directed evolution relieves product inhibition and confers in vivo function to a rationally designed tyrosine aminotransferase [O] . Rothman, Steven C., Voorhies, Mark, Kirsch, Jack F. 2004

机译：定向进化消除了产物抑制，并赋予了体内设计合理的酪氨酸转氨酶功能

Directed evolution: the 'rational' basis for 'irrational' design

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