...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Materials Science >Ultrasensitive aptamer-based biosensor for acetamiprid using tetrahedral DNA nanostructures
【24h】

Ultrasensitive aptamer-based biosensor for acetamiprid using tetrahedral DNA nanostructures

机译：使用四面体DNA纳米结构的acetamiprid基于超敏感的适体的生物传感器

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An electrochemical tetrahedral APT biosensor (Au-TAN biosensor) was successfully constructed, which had a stable structure and excellent performance using tetrahedral DNA nanomaterials (TDN) and Au nanoparticles (AuNPs) for the detection of acetamiprid. Specifically, we designed a partial paired complementary DNA (cDNA) of acetamiprid aptamer and inserted the cDNA together with the acetamiprid aptamer into four single-stranded DNA sequences forming each side of the TDN, modifying AuNPs to increase the current signal intensity, to assemble the Au-TAN. This proposed aptasensor was compared with ssAPT aptasensor and tetrahedral APT nanostructures (TAN) aptasensor, respectively. The results showed that the performance of the designed aptasensor was better than those of the above two aptasensors. The detection limit of the aptasensor was calculated to be 0.077 pM (S/N = 3), which was far lower than that of other methods that we compared. Under optimized conditions, the proposed aptasensor showed a wide linear detection range from 0.1 pM to 10 nM. The Au-TAN biosensor opens up new ideas for the construction of aptasensors and provides a new method for detection of small molecular substances, such as pesticides residues.
机译：成功构建了一种电化学四面体APT生物传感器（AU-TAN Biosensor），其具有稳定的结构和使用四面体DNA纳米材料（TDN）和Au纳米颗粒（AUNP）的优异性能，用于检测acetamiprid。具体地，我们设计了acetamiprid适体的部分成对互补DNA（cDNA），并将cDNA与acetamiprid适体一起插入四个单链DNA序列形成TDN的每一侧，改变AUNP以增加电流信号强度，以组装Au-Tan。将该提出的Aptasensor与SSAPT Aptasensor和四面体APT纳米结构（TAN）Aptasensor进行了比较。结果表明，设计的Aptasensor的性能优于上述两个Aptasensors的性能。 Aptasensor的检测限为0.077μm（s / n = 3），远低于我们比较的其他方法的pm（s / n = 3）。在优化条件下，所提出的aptasensor显示出宽的线性检测范围为0.1pm至10nm。 Au-Tan Biosensor开辟了建造Aptasensors的新想法，并提供了一种用于检测小分子物质的新方法，例如农药残留物。

著录项

  • 来源
    《Journal of Materials Science》 |2020年第33期|共13页
  • 作者

    Yao Y.; Wang G. X.; Shi X. J.; Li J. S.; Yang F. Z.; Cheng S. T.; Zhang H.; Dong H. W.; Guo Y. M.; Sun X.; Wu Y. X.;

  • 作者单位

    Shandong Univ Technol Sch Agr Engn &

    Food Sci 12 Zhangzhou Rd Zibo 255049 Peoples R China;

    Shandong Univ Technol Sch Agr Engn &

    Food Sci 12 Zhangzhou Rd Zibo 255049 Peoples R China;

    Shandong Univ Technol Sch Agr Engn &

    Food Sci 12 Zhangzhou Rd Zibo 255049 Peoples R China;

    Shandong Univ Technol Sch Agr Engn &

    Food Sci 12 Zhangzhou Rd Zibo 255049 Peoples R China;

    Shandong Univ Technol Sch Agr Engn &

    Food Sci 12 Zhangzhou Rd Zibo 255049 Peoples R China;

    Shandong Univ Technol Sch Agr Engn &

    Food Sci 12 Zhangzhou Rd Zibo 255049 Peoples R China;

    Shandong Univ Technol Sch Agr Engn &

    Food Sci 12 Zhangzhou Rd Zibo 255049 Peoples R China;

    Shandong Univ Technol Sch Agr Engn &

    Food Sci 12 Zhangzhou Rd Zibo 255049 Peoples R China;

    Shandong Univ Technol Sch Agr Engn &

    Food Sci 12 Zhangzhou Rd Zibo 255049 Peoples R China;

    Shandong Univ Technol Sch Agr Engn &

    Food Sci 12 Zhangzhou Rd Zibo 255049 Peoples R China;

    Shandong Lvdu Biotechnol Co LTD Binzhou 256600 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 工程材料学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号