• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Ultrafast redistribution of E. coli SSB along Long single-stranded DNA via intersegment transfer
【24h】

Ultrafast redistribution of E. coli SSB along Long single-stranded DNA via intersegment transfer

机译：超速再分布在长单链DNA的大肠杆菌SSB通过偏心转移

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Single-stranded DNA binding proteins (SSBs) selectively bind single-stranded DNA (ssDNA) and facilitate recruitment of additional proteins and enzymes to their sites of action on DNA. SSB can also locally diffuse on ssDNA, which allows it to quickly reposition itself while remaining bound to ssDNA. In this work, we used a hybrid instrument that combines single-molecule fluorescence and force spectroscopy to directly visualize the movement of Escherichia coli SSB on long polymeric ssDNA. Long ssDNA was synthesized without secondary structure that can hinder quantitative analysis of SSB movement. The apparent diffusion coefficient of E. coli SSB thus determined ranged from 70,000 to 170,000 nt2/s, which is at least 600 times higher than that determined from SSB diffusion on short ssDNA oligomers, and is within the range of values reported for protein diffusion on double-stranded DNA. Our work suggests that SSB can also migrate via a long-range intersegment transfer on long ssDNA. The force dependence of SSB movement on ssDNA further supports this interpretation.
机译：单链DNA结合蛋白（SSBS）选择性地结合单链DNA（SSDNA）并促进募集另外的蛋白质和酶对其对DNA的作用部位。 SSB还可以在SSDNA上局部地区扩散，这允许其快速重新定位，同时留在绑定到SSDNA。在这项工作中，我们使用了一种混合仪器，所述混合仪器结合单分子荧光和力光谱，直接可视化大肠杆菌SSB在长聚合物SSDNA上的运动。在没有二级结构的情况下合成LONG SSDNA，可以妨碍SSB运动的定量分析。由此确定的大肠杆菌SSB的表观扩散系数范围为70,000至170,000nt2 / s，其比在短的SSDNA低聚物上从SSB扩散确定的高度高至少600倍，并且在报告的蛋白质扩散的值范围内双链DNA。我们的工作表明，SSB还可以通过Long SSDNA的远程字体传输迁移。 SSB运动对SSDNA的力依赖性进一步支持这种解释。

著录项

  • 来源
    《Journal of Molecular Biology》 |2014年第13期|共9页
  • 作者

    LeeK.S.; MarcielA.B.; KozlovA.G.; SchroederC.M.; LohmanT.M.; HaT.;

  • 作者单位

    Department of Physics Center for Physics in Living Cells University of Illinois at Urbana;

    Center for Biophysics and Computational Biology University of Illinois at Urbana-Champaign IL;

    Department of Biochemistry and Molecular Biophysics Washington University School of Medicine 660;

    Center for Biophysics and Computational Biology University of Illinois at Urbana-Champaign IL;

    Department of Biochemistry and Molecular Biophysics Washington University School of Medicine 660;

    Department of Physics Center for Physics in Living Cells University of Illinois at Urbana;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子生物学;
  • 关键词

    optical tweezer; single-stranded DNA; single-stranded DNA binding protein;

    机译：光学镊子;单链DNA;单链DNA结合蛋白;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号