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首页> 外文期刊>Journal of Molecular Biology >SRP, FtsY, DnaK and YidC Are Required for the Biogenesis of the E. coli Tail-Anchored Membrane Proteins DjIC and Flk
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SRP, FtsY, DnaK and YidC Are Required for the Biogenesis of the E. coli Tail-Anchored Membrane Proteins DjIC and Flk

机译：E. Coli尾锚膜蛋白质和FLK的生物发生需要SRP，FTSY，DNAK和YIDC

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Tail-anchored membrane proteins (TAMPs) are relatively simple membrane proteins characterized by a single transmembrane domain (TMD) at their C-terminus. Consequently, the hydrophobic TMD, which acts as a subcellular targeting signal, emerges from the ribosome only after termination of translation precluding canonical co-translational targeting and membrane insertion. In contrast to the well-studied eukaryotic TAMPs, surprisingly little is known about the cellular components that facilitate the biogenesis of bacterial TAMPs. In this study, we identify DjIC and Flk as bona fide Escherichia co/iTAMPs and show that their TMDs are necessary and sufficient for authentic membrane targeting of the fluorescent reporter mNeonGreen. Using strains conditional for the expression of known E. coli membrane targeting and insertion factors, we demonstrate that the signal recognition particle (SRP), its receptor FtsY, the chaperone DnaK and insertase YidC are each required for efficient membrane localization of both TAMPs. A close association between the TMD of DjIC and Flk with both the Ffh subunit of SRP and YidC was confirmed by site-directed in vivo photo-crosslinking. In addition, our data suggest that the hydrophobicity of the TMD correlates with the dependency on SRP for efficient targeting. (C) 2017 The Authors. Published by Elsevier Ltd.
机译：尾锚膜蛋白（罐）是在其C-末端的单个跨膜结构域（TMD）的相对简单的膜蛋白。因此，作为亚细胞靶向信号的疏水性TMD仅在核糖体之后从核糖体中出现，终止于翻译预定典型的典型协同靶向靶向和膜插入。与良好的学习真核棉酒相比，令人惊讶地对促进细菌罐的生物发生的细胞组分令人惊讶的是。在这项研究中，我们将DJIC和FLK识别为BONA FIDE ESCHERICHIA CO / itamps，并表明它们的TMD是必要的，并且足以用于荧光报告器Mneongreen的真正膜靶向。使用菌株条件用于表达已知的大肠杆菌膜靶向和插入因子，我们证明了信号识别粒子（SRP），其受体FTSY，伴侣DNAK和渗透酶YIDC各自需要两种棉隙的有效膜定位所需的。通过SRP和YIDC的FFH亚基与SRP和YIDC的FFH亚基之间的TMD之间的紧密关联通过在Vivo Photo-Crosslinching中进行了定点。此外，我们的数据表明TMD的疏水性与对SRP的依赖性相关，以便有效靶向。 （c）2017年作者。 elsevier有限公司出版

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