• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Molecular Determinants for 23S rRNA Recognition and Modification by the E-coli Pseudouridine Synthase RIuE
【24h】

Molecular Determinants for 23S rRNA Recognition and Modification by the E-coli Pseudouridine Synthase RIuE

机译：通过E-COLI假尿苷合酶Riue的23s RRNA识别和修饰的分子决定簇

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The isomerization of uridine to pseudouridine is the most common type of RNA modification found in RNAs across all domains of life and is performed by RNA-dependent and RNA-independent enzymes. The Escherichia coli pseudouridine synthase RIuE acts as a stand-alone, highly specific enzyme forming the universally conserved pseudouridine at position 2457, located in helix 89 (H89) of the 23S rRNA in the peptidyltransferase center. Here, we conduct a detailed structure function analysis to determine the structural elements both in RIuE and in 23S rRNA required for RNA protein interaction and pseudouridine formation. We determined that RIuE recognizes a large part of 23S rRNA comprising both H89 and the single-stranded flanking regions which explains the high substrate specificity of RIuE. Within RIuE, the target RNA is recognized through sequence-specific contacts with loop L7-8 as well as interactions with loop L1-2 and the flexible N-terminal region. We demonstrate that RIuE is a faster pseudouridine synthase than other enzymes which likely enables it to act in the early stages of ribosome formation. In summary, our biochemical characterization of RIuE provides detailed insight into the molecular mechanism of RIuE forming a highly conserved pseudouridine during ribosome biogenesis. (C) 2018 The Authors. Published by Elsevier Ltd.
机译：尿苷至假尿苷的异构化是在所有寿讯结构域中发现的RNA中最常见的RNA改性，并且由RNA依赖性和RNA无关的酶进行。大肠杆菌假尿苷合酶riue作为独立的高度特异性酶，其形成在肽基丙烷酶中心23s rRNA的Helix 89（H89）中的普遍保守的假尿苷。这里，我们进行详细的结构功能分析，以确定RNA蛋白相互作用和假尿苷形成所需的速度和23S rRNA中的结构元素。我们确定Riue识别23s rRNA的大部分，包括H89和单链侧翼区域，所述单链侧翼区域解释了Riue的高底物特异性。在Riue内，通过与环L7-8的序列特异性触点以及与环L1-2和柔性N末端区域的相互作用来识别靶RNA。我们证明Riue是比其他酶更快的假尿苷合酶，这可能使其能够在核糖体形成的早期阶段起作用。总之，我们的Riue的生化特征提供了对核糖体生物发生期间形成高度保守的假尿苷的riue的分子机制的详细洞察。 （c）2018年作者。 elsevier有限公司出版

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号