• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Processive Recoding and Metazoan Evolution of Selenoprotein P: Up to 132 UGAs in Molluscs
【24h】

Processive Recoding and Metazoan Evolution of Selenoprotein P: Up to 132 UGAs in Molluscs

机译：加工重新编码和硒蛋白P的甲烷蛋白酶的进化：MOLLUSC中最多132例UGA

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Selenoproteins typically contain a single selenocysteine, the 21st amino acid, encoded by a context-redefined UGA. However, human selenoprotein P (SelenoP) has a redox-functioning selenocysteine in its N-terminal domain and nine selenium transporter-functioning selenocysteines in its C-terminal domain. Here we show that diverse SelenoP genes are present across metazoa with highly variable numbers of Sec-UGAs, ranging from a single UGA in certain insects, to 9 in common spider, and up to 132 in bivalve molluscs. SelenoP genes were shaped by a dynamic evolutionary process linked to selenium usage. Gene evolution featured modular expansions of an ancestral multi-Sec domain, which led to particularly Sec-rich SelenoP proteins in many aquatic organisms. We focused on molluscs, and chose Pacific oyster Magallana gigas as experimental model. We show that oyster SelenoP mRNA with 46 UGAs is translated full-length in vivo. Ribosome profiling indicates that selenocysteine specification occurs with similar to 5% efficiency at UGA1 and approaches 100% efficiency at distal 3' UGAs. We report genetic elements relevant to its expression, including a leader open reading frame and an RNA structure overlapping the initiation codon that modulates ribosome progression in a selenium-dependent manner. Unlike their mammalian counterparts, the two SECIS elements in oyster SelenoP (3'UTR recoding elements) do not show functional differentiation in vitro. Oysters can increase their tissue selenium level up to 50-fold upon supplementation, which also results in extensive changes in selenoprotein expression. (C) 2019 The Author(s). Published by Elsevier Ltd.
机译：硒蛋白通常包含单个硒代半胱氨酸，第21氨基酸，由上下文重新定义的UGA编码。然而，人硒蛋白P（SELENOP）在其N-末端结构域中具有氧化还原功能硒细胞，其C末端结构域中的九个硒转运蛋白能量。在这里，我们表明，各种Selenop基因存在于常量数量的SEC-UGA中，从某些昆虫中的单个UGA，常见蜘蛛中的9个，并且在双戊丝Mollusc中的比例为9。通过与硒使用的动态进化方法形成硒基因。基因进化功能的祖先多秒域，从而导致秒，尤其是丰富的蛋白质SelenoP在许多水生生物的模块化扩展。我们专注于软体动物，并选择太平洋牡蛎马格拉纳吉斯作为实验模型。我们表明，具有46个UGA的Oyster Selenop mRNA在体内翻译全长。核糖体分析表明，在UGA1的效率类似于5％效率，在远端3'UGA下接近硒的细胞内容。我们报告与其表达相关的遗传元素，包括引导开放阅读框和重叠的引发密码子的RNA结构以依赖硒的方式调节核糖体进展。与他们的哺乳动物同行不同，牡蛎SELENOP（3'UTR读码元素）中的两个SECIE元件在体外没有显示功能分化。牡蛎在补充后可以将组织硒水平增加至50倍，这也导致硒代蛋白表达的广泛变化。 （c）2019年作者。 elsevier有限公司出版

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号