• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Myeloid Leukemia Factor Acts in a Chaperone Complex to Regulate Transcription Factor Stability and Gene Expression
【24h】

Myeloid Leukemia Factor Acts in a Chaperone Complex to Regulate Transcription Factor Stability and Gene Expression

机译：骨髓白血病因子在伴侣络合物中起作用以调节转录因子稳定性和基因表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mutations that affect myelodysplasia/myeloid leukemia factor (MLF) proteins are associated with leukemia and several other cancers. However, with no strong homology to other proteins of known function, the role of MLF proteins in the cell has remained elusive. Here, we describe a proteomics approach that identifies MLF as a member of a nuclear chaperone complex containing a DnaJ protein, BCL2-associated anthanogene 2, and Hsc70. This complex associates with chromatin and regulates the expression of target genes. The MLF complex is bound to sites of nucleosome depletion and sites containing active chromatin marks (e.g., H3K4me3 and H3K4me1). Hence, MLF binding is enriched at promoters and enhancers. Additionally, the MLF-chaperone complex functions to regulate transcription factor stability, including the RUNX transcription factor involved in hematopoiesis. Although Hsc70 and other co-chaperones have been shown to play a role in nuclear translocation of a variety of proteins including transcription factors, our findings suggest that MLF and the associated co-chaperones play a direct role in modulating gene transcription. (C) 2016 Elsevier Ltd. All rights reserved.
机译：影响体育细胞膜/髓性白血病因子（MLF）蛋白质的突变与白血病和其他几种癌症有关。然而，对于已知功能的其他蛋白质没有强烈的同源性，MLF蛋白在细胞中的作用仍然难以捉摸。这里，我们描述了一种蛋白质组学方法，其将MLF识别为含有DNAJ蛋白，BCL2相关的沙氨基2和HSC70的核伴侣络合物的成员。这种复杂的与染色质辅助助致并调节靶基因的表达。 MLF复合物与含有活性染色质标记的核心耗竭的位点（例如，H3K4ME3和H3K4ME1）结合。因此，MLF结合在促进剂和增强剂处富集。另外，MLF-伴侣络合物的作用以调节转录因子稳定性，包括血液缺陷中所涉及的RUNX转录因子。虽然HSC70和其他共伴侣已经显示出在包括转录因子的各种蛋白质的核易位中发挥作用，但我们的研究结果表明，MLF和相关的共伴侣在调节基因转录中起到直接作用。 （c）2016 Elsevier有限公司保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号