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首页> 外文期刊>Journal of Molecular Biology >Comprehensive Identification of Nuclear and Cytoplasmic TNRC6A-Associating Proteins
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Comprehensive Identification of Nuclear and Cytoplasmic TNRC6A-Associating Proteins

机译：核和细胞质TNRC6A关联蛋白的综合鉴定

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Abstract Trinucleotide repeat-containing gene 6A protein (TNRC6A) is an essential protein for microRNA-mediated gene silencing. TNRC6A functions in the cytoplasm as a platform protein interacting with Argonaute protein, on which microRNA is loaded for RNA silencing, and decapping enzymes or deadenylation protein complexes to induce mRNA degradation. We previously revealed that TNRC6A shuttles between the cytoplasm and nucleus. However, the function of TNRC6A in the nucleus is unclear. Here, we performed a comprehensive identification of the nuclear and cytoplasmic interacting proteins of TNRC6A protein by mass spectrometry and identified multiple proteins involved in the nuclear and cytoplasmic complexes. We found that many RNA degradation pathway proteins were involved in both nuclear and cytoplasmic TNRC6A complexes, suggesting that RNA silencing may occur via TNRC6A in both nucleus and cytoplasm or that they were involved in other important function in the nucleus. Furthermore, proteins identified in the nuclear TNRC6A complex were categorized into the spliceosomal pathway. This may mean that TNRC6A regulates splicing in the nucleus. In contrast, pathogen infection- and RNA transport-associated proteins were identified in the cytoplasmic TNRC6A complex. Thus, TNRC6A may be also involved in these pathways in the cytoplasm. Graphical Abstract Display Omitted Highlights ? Mass spectrometry analysis of the nuclear or cytoplasmic TNRC6A-interacting proteins ? Both nuclear and cytoplasmic TNRC6A proteins interact with proteins involved in RNA degradation pathway. ? The nuclear TNRC6A specifically interacted with proteins involved in spliceosome. ? The cytoplasmic TNRC6A specifically interacted with proteins involved in pathogen infection or RNA transport.
机译：摘要含杂交重复的基因6a蛋白（TNRC6A）是MicroRNA介导的基因沉默的必需蛋白。 TNRC6A在细胞质中的作用作为与Argonaute蛋白相互作用的平台蛋白质，在该蛋白质中加载MicroRNA用于RNA沉默，并折断酶或硬烯酰化蛋白质复合物以诱导mRNA降解。我们以前透露，细胞质和核之间的TNRC6A梭。然而，核中TNRC6A的功能尚不清楚。在这里，我们通过质谱法对TNRC6A蛋白的核和细胞质相互作用蛋白进行了综合鉴定，并鉴定了核和细胞质复合物中的多种蛋白质。我们发现许多RNA降解途径蛋白涉及核和细胞质TNRC6A络合物，表明RNA沉默可以通过核和细胞质中的TNRC6A发生，或者它们参与细胞核中的其他重要功能。此外，在核TNRC6A络合物中鉴定的蛋白质被分类为抗乳溶酶体途径。这可能意味着TNRC6A调节核中的剪接。相反，在细胞质TNRC6A络合物中鉴定了病原体感染和RNA传输相关的蛋白质。因此，TNRC6A也可以参与细胞质中的这些途径。图形抽象显示省略了亮点？核或细胞质TNRC6A相互作用蛋白的质谱分析？核和细胞质TNRC6A蛋白质均与参与RNA降解途径的蛋白质相互作用。还是核TNRC6a特别与粘合剂组合的蛋白质相互作用。还是细胞质TNRC6A与涉及病原体感染或RNA转运的蛋白质特异性相互作用。

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