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首页> 外文期刊>Biosciences Biotechnology Research Asia >Heavy Metal Binding and Accumulation of Genetically Engineered E. coli Harboring the CXXC Motif and Histidine Rich Motif Fusion Proteins
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Heavy Metal Binding and Accumulation of Genetically Engineered E. coli Harboring the CXXC Motif and Histidine Rich Motif Fusion Proteins

机译：具有CXXC母题和组氨酸丰富的母题融合蛋白的基因工程大肠杆菌的重金属结合和积累

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摘要

Bioremediation is a biological method for removing heavy metals from the environment. Heavy metal binding proteins have been identified in various organisms and have been used to study bioremediation. CXXC and histidine peptide rich motifs are metal binding domains found in proteins of all living organisms. Genes encoding the metal binding domain CXXC motif from the mcsA gene and histidine peptide rich motifs from the copA gene of S. aureus were used to construct 3 recombinant proteins containing various metal binding domains, C4. C8 and C4His recombinant proteins. C4 and C8 recombinant proteins contain four and eight domains of CXXC motifs, respectively. C4His recombinant protein contains four domains of the CXXC motif fused with a histidine-rich metal binding domain. Recombinant proteins were tested for metal binding using IAA chromatography. C4 and C8 recombinant proteins bound Cu~(2+), Zn~(2+), Cd~(2+)and Co2+ , whereas, the C4His recombinant protein did not bind to any heavy metal tested. E. coli expressed with C4 or C8 recombinant proteins showed increased resistance to Cu~(2+) and Cd~(2+). E. coli expressed with C4His recombinant proteins showed increased resistance to Cd~(2+). E. coli expressed with C4, C8 or C4His recombinant proteins were tested for intracellular bioaccumulation under various heavy metal conditions. The results showed E. co/iexpressed C8 recombinant protein had the highest Cu~(2+) and Cd~(2+) intracellular bioaccumulation above control. This study shows that metal binding domain recombinant proteins can effectively bind and accumulate various types of heavy metals and are good potential tool for studying bioremediation.
机译：生物修复是一种从环境中去除重金属的生物方法。重金属结合蛋白已在各种生物中得到鉴定，并已用于研究生物修复。 CXXC和组氨酸富含肽的基序是在所有活生物体蛋白质中发现的金属结合域。使用来自金黄色葡萄球菌的mcsA基因编码金属结合域CXXC基序的基因和来自金黄色葡萄球菌copA基因的富含组氨酸肽基序的基因来构建3个包含各种金属结合域C4的重组蛋白。 C8和C4His重组蛋白。 C4和C8重组蛋白分别包含CXXC基序的四个和八个域。 C4His重组蛋白包含与富含组氨酸的金属结合域融合的CXXC基序的四个域。使用IAA色谱法测试重组蛋白的金属结合。 C4和C8重组蛋白与Cu〜（2 +），Zn〜（2 +），Cd〜（2+）和Co2 +结合，而C4His重组蛋白不与任何重金属结合。用C4或C8重组蛋白表达的大肠杆菌对Cu〜（2+）和Cd〜（2+）的抗性增强。用C4His重组蛋白表达的大肠杆菌对Cd〜（2+）的抗性增强。测试了在各种重金属条件下用C4，C8或C4His重组蛋白表达的大肠杆菌的细胞内生物蓄积性。结果表明，大肠杆菌表达的C8重组蛋白具有高于对照的最高Cu〜（2+）和Cd〜（2+）细胞内生物蓄积性。这项研究表明金属结合域重组蛋白可以有效地结合和积累各种类型的重金属，并且是研究生物修复的良好潜在工具。

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