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首页> 外文期刊>Biosciences Biotechnology Research Asia >Preparation of Maltotriose Syrup from Microbial Pullulan by using Pullulanase Enzyme
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Preparation of Maltotriose Syrup from Microbial Pullulan by using Pullulanase Enzyme

机译：支链淀粉酶从微生物支链淀粉制备麦芽三糖浆

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摘要

The present work deals with the preparation and single point optimization of maltotriose syrup prepared from microbial pullulan. The enzyme pullulanase was used in this process for hydrolysis of pullulan. Production of pullulan was carried out in a shake flask fermenter with isopropanol precipitation. Various parameters like time, temperature, pH, amount of ethanol and effect of amount of enzyme on the hydrolysis of pullulan were optimized by single point optimization studies. The optimized values for the process parameters were 8h, 46°C, 5.5, 6 (v/v) and 10 ASPU/g for time of hydrolysis, temperature, pH , amount of ethanol and amount of pullulanase enzyme respectively. The results obtained from HPLC, confirmed that major component present in the hydrolysate were composed of maltotriose. Results of experiments indicated that this was a promising way of preparation of maltotriose.
机译：目前的工作涉及由微生物支链淀粉制备的麦芽三糖糖浆的制备和单点优化。支链淀粉酶在该过程中用于支链淀粉的水解。支链淀粉的生产在带有异丙醇沉淀的摇瓶发酵罐中进行。通过单点优化研究，优化了时间，温度，pH，乙醇量和酶用量对支链淀粉水解的各种参数。对于水解时间，温度，pH，乙醇量和支链淀粉酶的量，工艺参数的最佳值分别为8h，46℃，5.5、6（v / v）和10 ASPU / g。从HPLC获得的结果证实了存在于水解产物中的主要成分是麦芽三糖。实验结果表明，这是制备麦芽三糖的有前途的方法。

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