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首页> 外文期刊>Journal of Neurophysiology >Optogenetic and transcriptomic interrogation of enhanced muscle function in the paralyzed mouse whisker pad
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Optogenetic and transcriptomic interrogation of enhanced muscle function in the paralyzed mouse whisker pad

机译：瘫痪鼠须垫中增强肌肉功能的光学和转录组询问

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The functional state of denervated muscle is a critical factor in the ability to restore movement after injury-or disease-related paralysis. Here we used peripheral optogenetic stimulation and transcriptome profiling in the mouse whisker system to investigate the time course of changes in neuromuscular function following complete unilateral facial nerve transection. While most skeletal muscles rapidly lose functionality after lower motor neuron denervation, optogenetic muscle stimulation of the paralyzed whisker pad revealed sustained increases in the sensitivity, velocity, and amplitude of whisker movements, and reduced fatigability, starting 48 h after denervation. RNA-seq analysis showed distinct regulation of multiple gene families in denervated whisker pad muscles compared with the atrophy-prone soleus, including prominent changes in ion channels and contractile fibers. Together, our results define the unique functional and transcriptomic landscape of denervated facial muscles and have general implications for restoring movement after neuromuscular injury or disease.
机译：虚脱肌的功能状态是损伤或疾病相关瘫痪后恢复运动的关键因素。在这里，我们在小鼠晶须系统中使用了外周的致敏刺激和转录组分析，研究了完全单侧面部神经横断面后神经肌肉功能的变化时间过程。虽然大多数骨骼肌快速丧失较低运动后神经元后的功能性，但瘫痪的晶须垫的致敏肌刺激透露持续增加晶须运动的敏感，速度和振幅，并降低疲劳性，在后脱落后开始48小时。 RNA-SEQ分析表明，与萎缩的易于单独的单独组（包括离子通道和收缩纤维）的突出变化，显示出在方便的晶须垫中多种基因家族的明显调节。我们的结果共同定义了神经肌肉损伤或疾病后恢复运动的独特功能和转录组景观。

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