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首页> 外文期刊>Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis >A comprehensive LC-MS/MS method validation for the quantitative investigation of 37 fingerprint phytochemicals in Achillea species: A detailed examination of A. coarctata and A. monocephala
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A comprehensive LC-MS/MS method validation for the quantitative investigation of 37 fingerprint phytochemicals in Achillea species: A detailed examination of A. coarctata and A. monocephala

机译：综合的LC-MS / MS方法验证Achilla植物37种指纹植物化学症的定量调查：对A.Coarctata和A.单肉藻菌的详细检查

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The current study aims to optimize and validate a comprehensive LC-MS/MS method for the quantification of 37 phytochemicals (15 phenolic acids, 17 flavonoids, 3 non-phenolic organic acids, 1 phenolic aldehyde and 1 benzopyrene) in Achillea species. Though Achillea species were chosen as real life samples, the current method is applicable to a wide range of plant species. The developed method was fully validated in terms of linearity, accuracy (recovery), inter-day and intra-day precision (repeatability), limits of detection and quantification (LOD/LOQ) and relative standard uncertainty (U% at 95% confidence level (k = 2)). Reversed-phase ultrahigh performance liquid chromatography was optimized to achive optimum separation for 37 phytochemical compounds and to overcome the suppression effects. MS detection was performed using a triple quadrupole mass spectrometer and negative or positive ionization modes were optimized for each analyte. Multiple reaction monitoring (MRM) was used to quantify the analytes, related molecular ions and transition ions were optimized. Phytochemical screening of ethanol and methanol-chloroform extracts of root and aerial parts of A. coarctata and A. monocephala were performed by using the developed and validated LC-MS/MS method. Root and aerial parts of both species have considerable amounts of certain phenolic-nonphenolic acids (quinic, malic, fumaric, chlorogenic and vanillic acids) and flavonoids (rutin, hesperidin, isoquercitrin, apigetrin, luteolin, apigenin). Additionally, total phenolic and flavonoid amounts, antioxidant (DPPH free radical scavenging assay, ABTS radical cation decolorization assay, beta-carotene lipid peroxidation test system and CUPRAC cupper reduction capacity methods), anticholinesterase, tyrosinase, urease inhibition and cytotoxic activities (on HeLa (Human Cervical Carcinoma Cell Line) of A. coarctata and A. monocephala were also investigated. It has been determined that the studied Achillea species, that are rich in total phenolic-flavonoid and chlorogenic acid contents, have high antioxidant and cytotoxic potential at the same time. According to the results of LC-MS/MS, antioxidant and cytotoxic activity studies, after detailed chemical investigation and toxicity studies on these species, A. coarctata and A. monocephala may be promoted as promising sources of natural agents and used for the development of nutraceuticals or functional food ingredients in future. (C) 2018 Elsevier B.V. All rights reserved.
机译：目前的研究旨在优化和验证Achillaea种类中37种植物化学物质（15个酚酸，17种黄酮，3个非酚醛糖，1个苯酚醛和1个苯并醛）的综合LC-MS / MS方法。虽然achillea种被选择为现实生活样本，但目前的方法适用于各种植物物种。开发的方法在线性，准确度（恢复），日内和日内的精度（可重复性），检测和定量限制（LOD / LOQ）和相对标准不确定性（U％，在95％置信水平上（k = 2））。反相超高效液相色谱经过优化，以实现37种植物化合物的最佳分离，并克服抑制效果。使用三重四极杆质谱仪进行MS检测，对每个分析物进行优化负离子化或阳性电离模式。多次反应监测（MRM）用于量化分析物，优化相关的分子离子和过渡离子。通过使用显影和验证的LC-MS / MS方法进行乙醇的乙醇和甲醇 - 氯仿萃取物的乙醇和甲醇 - 氯仿提取物。两种物种的根和空中部位具有相当大量的酚醛 - 非烯醇（奎因，麦芽，富马酸，香草酸）和黄酮类化合物（Rutin，Herperidin，异喹啉，Apigetrin，Luteolin，Apigenin）。另外，总酚类和黄酮类化合物量，抗氧化剂（DPPH自由基清除测定，ABTS自由基阳离子脱色测定，β-胡萝卜素脂质过氧化试验系统和Cuprac Chopper降低能力方法），抗胆碱酯酶，酪氨酸酶，脲酶抑制和细胞毒性活性（在Hela（还研究了A. coarctata和A.单肉宫的人颈癌细胞系。已经确定了富含酚类黄酮和绿原酸含量丰富的研究症状，具有高抗氧化剂和细胞毒性潜力时间。根据LC-MS / MS，抗氧化剂和细胞毒性活性研究的结果，在详细的化学研究和对这些物种的毒性研究之后，A.Coarctata和A.单肉藻可以作为天然代理的有希望的来源促进并用于未来的营养保健品或功能性食品的开发。（c）2018年Elsevier BV保留所有权利。

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