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首页> 外文期刊>Journal of Physics, D. Applied Physics: A Europhysics Journal >Between life and death: strategies to reduce phototoxicity in super-resolution microscopy
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Between life and death: strategies to reduce phototoxicity in super-resolution microscopy

机译:生与死之间的:减少超分辨率显微镜中光毒性的策略

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摘要

Super-resolution microscopy (SRM) enables non-invasive, molecule-specific imaging of the internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its major limitations is the requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits the capacity for live-cell observations, particularly for extended periods of time. Here, we give an overview of new developments in hardware, software and probe chemistry aiming to reduce phototoxicity. Additionally, we discuss how the choice of biological model and sample environment impacts the capacity for live-cell observations.
机译:超分辨率显微镜(SRM)使得具有副衍射限制空间分辨率的内部结构和细胞的内部结构和动态的非侵入性,分子特异性成像。 其主要限制之一是要求高强度照明,产生相当大的细胞光毒性。 该因素大大限制了活细胞观测的能力,特别是在延长的时间段内。 在这里,我们概述了旨在减少光毒性的硬件,软件和探针化学的新发展。 此外,我们讨论了生物学模型和样本环境的选择如何影响活细胞观测的能力。

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