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首页> 外文期刊>Journal of Physics, D. Applied Physics: A Europhysics Journal >High photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy
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High photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy

机译：高光子计数率提高超分辨率荧光波动光谱的质量

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摘要

Probing the diffusion of molecules has become a routine measurement across the life sciences, chemistry and physics. It provides valuable insights into reaction dynamics, oligomerisation, molecular (re-)organisation or cellular heterogeneities. Fluorescence correlation spectroscopy (FCS) is one of the widely applied techniques to determine diffusion dynamics in two and three dimensions. This technique relies on the temporal autocorrelation of intensity fluctuations but recording these fluctuations has thus far been limited by the detection electronics, which could not efficiently and accurately time-tag photons at high count rates. This has until now restricted the range of measurable dye concentrations, as well as the data quality of the FCS recordings, especially in combination with super-resolution stimulated emission depletion (STED) nanoscopy.
机译：探测分子的扩散已成为跨生命科学，化学和物理学的常规测量。 它为反应动力学，低聚，分子（重新均匀）或细胞异质性提供了有价值的见解。 荧光相关光谱（FCS）是广泛应用的技术之一，以确定两和三维的扩散动力学。 该技术依赖于强度波动的时间自相关，但是记录这些波动的迄今为止受到检测电子器件的限制，其在高计数速率下无法有效和准确地时间标签光子。 这直到现在限制了可测量的染料浓度的范围，以及FCS记录的数据质量，特别是与超分辨率刺激排放耗尽（STED）纳米镜的组合。

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