Compressed Hadamard microscopy for high-speed optically sectioned neuronal activity recordings

Parot Vicente J.; Sing-Long Carlos; Adam Yoav; Bohm Urs L.; Fan Linlin Z.; Farhi Samouil L.; Cohen Adam E.

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Compressed Hadamard microscopy for high-speed optically sectioned neuronal activity recordings

机译：压缩Hadamard显微镜用于高速光学切片的神经元活动记录

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Structured illumination microscopic achieve optical sectioning via differential modulation of in-focus and out-of-focus contributions to an image. Multiple wide-field camera images are analyzed to recreate an optical section. The requirement for multiple camera frames per image entails a loss of temporal resolution compared to conventional wide-field imaging. Here we describe a computational structured illumination imaging scheme, compressed Hadamard imaging, which achieves simultaneously high spatial and temporal resolution for optical sectioning of 3D samples with low-rank dynamics (e.g. neurons labeled with fluorescent activity reporters). We validate the technique with numerical simulations, and then illustrate with wide-area optically sectioned recordings of membrane voltage dynamics in mouse neurons in an acute brain slice and of calcium dynamics in zebrafish brain in vivo.

机译：结构化照明显微镜通过差分调制和焦点贡献对图像的差分调制来实现光学切片。分析多个宽场摄像机图像以重建光学部分。与传统的宽场成像相比，每个图像的多个相机帧的要求需要丢失时间分辨率。在这里，我们描述了一种计算结构化照明成像方案，压缩的Hadamard成像，其同时实现了具有低秩动力学的3D样品的光学切片的高空间和时间分辨率（例如，用荧光活动记录器标记的神经元）。我们用数值模拟验证了该技术，然后用急性脑切片中的小鼠神经元中的膜电压动力学和体内斑马鱼脑中的钙动力学中的膜电压动力学的广域光学切片录制。

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《Journal of Physics, D. Applied Physics: A Europhysics Journal》 |2019年第14期|共15页
作者
Parot Vicente J.; Sing-Long Carlos; Adam Yoav; Bohm Urs L.; Fan Linlin Z.; Farhi Samouil L.; Cohen Adam E.;
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作者单位

Harvard Univ Biophys Program Cambridge MA 02138 USA;

Pontificia Univ Catolica Chile Sch Engn Inst Math &

Computat Engn Santiago Chile;

Harvard Univ Dept Chem &

Chem Biol Cambridge MA 02138 USA;

Harvard Univ Dept Chem &

Chem Biol Cambridge MA 02138 USA;

Harvard Univ Dept Chem &

Chem Biol Cambridge MA 02138 USA;

Harvard Univ Dept Chem &

Chem Biol Cambridge MA 02138 USA;

Harvard Univ Dept Chem &

Chem Biol Cambridge MA 02138 USA;

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原文格式 PDF
正文语种 eng
中图分类应用物理学;
关键词
optical sectioning; neuronal activity recording; microscopy;

机译：光学切片;神经元活动记录;显微镜;

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专利

1. Compressed Hadamard microscopy for high-speed optically sectioned neuronal activity recordings [J] . Parot Vicente J., Sing-Long Carlos, Adam Yoav, Journal of Physics, D. Applied Physics: A Europhysics Journal . 2019,第14期

机译：压缩Hadamard显微镜用于高速光学切片的神经元活动记录
2. Overcoming photodamage in second-harmonic generation microscopy: Real-time optical recording of neuronal action potentials [J] . L. Sacconi, D. A. Dombeck, W. W. Webb Proceedings of the National Academy of Sciences of the United States of America . 2006,第9期

机译：克服二次谐波显微镜中的光损伤：实时光学记录神经元动作电位
3. High-speed label-free two-photon fluorescence microscopy of metabolic transients during neuronal activity [J] . Andrew J. Bower, Carlos Renteria, Joanne Li, Applied Physics Letters . 2021,第8期

机译：神经元活性期间代谢瞬态的高速无标签的两光子荧光显微镜
4. Fast Three-Dimensional Random Access Multi-Photon Microscopy for Functional Recording of Neuronal Activity [C] . Duemani Reddy, Peter Saggau Confocal, Multiphoton, and Nonlinear Microscopic Imaging III; Progress in Biomedical Optics and Imaging; vol.8 no.43; Proceedings of SPIE-The International Society for Optical Engineering; vol.6630 . 2007

机译：快速三维随机访问多光子显微镜用于神经元活动的功能记录。
5. Innovations of wide-field optical-sectioning fluorescence microscopy: toward high-speed volumetric bio-imaging with simplicity. [D] . Yu, Jiun-Yann. 2014

机译：宽视野光学切片荧光显微镜的创新：简便实现高速体积生物成像。
6. High-speed random-access fluorescence microscopy: I. High-resolution optical recording with voltage-sensitive dyes and ion indicators. [O] . A Bullen, S S Patel, P Saggau 1997

机译：高速随机存取荧光显微镜：I.带有压敏染料和离子指示剂的高分辨率光学记录。
7. High-speed, random-access fluorescence microscopy: I. High-resolution optical recording with voltage-sensitive dyes and ion indicators. [O] . Bullen, A, Patel, S S, Saggau, P 1997

机译：高速，随机存取荧光显微镜：I.带有压敏染料和离子指示剂的高分辨率光学记录。

Compressed Hadamard microscopy for high-speed optically sectioned neuronal activity recordings

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