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首页> 外文期刊>Journal of Physics, D. Applied Physics: A Europhysics Journal >Confocal Raman microscopy supported by optical clearing treatment of the skin-influence on collagen hydration
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Confocal Raman microscopy supported by optical clearing treatment of the skin-influence on collagen hydration

机译：Concocal拉曼显微镜通过光学清除治疗皮肤影响对胶原蛋白水合的影响

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摘要

Confocal Raman microscopy (CRM) is employed to study the skin physiology, drug permeation and skin disease monitoring. In order to increase the depth of investigations, the effect of optical clearing was observed on porcine ear skin ex vivo. The optical clearing agents (OCAs) glycerol and iohexol (Omnipaque (TM)) were applied to the porcine ear skin and investigated by CRM after 30 and 60 min of treatment. The extent of optical clearing by utilizing concentrations of 70% glycerol and 100% Omnipaque (TM) was evaluated. The intensity of the skin-related Raman peaks significantly increased starting from the depth 160 mu m for Omnipaque (TM) and 40 mu m for glycerol (p <= 0.05) after 60 min of treatment. The OCAs' influence on the collagen hydration in the deep-located dermis was investigated. Both OCAs induce skin dehydration, but the effect of glycerol treatment (30 min and 60 min) is stronger. The obtained results demonstrate that with increasing the treatment time, both glycerol and Omnipaque (TM) solutions improve the optical clearing of porcine skin making the deep-located dermal regions able for investigations. At the used concentrations and time intervals, glycerol is more effective than Omnipaque (TM). However, Omnipaque (TM) is more promising than glycerol for future in vivo applications as it is an already approved pharmaceutic substance without any known impact on the skin structure.
机译：共焦拉曼显微镜（CRM）用于研究皮肤生理学，药物渗透和皮肤病监测。为了提高研究深度，在猪耳肌外，观察到光学清除的效果。将光学清除剂（OCAS）甘油和IOHEXOL（OMNIPAQUE（TM））施用于猪耳皮，并在30分钟后通过CRM进行研究。通过利用70％甘油和100％Omnipaque（TM）来评价光学清除程度。皮肤相关拉曼峰的强度从在治疗60分钟后的甘油（TM）和40μm用于甘油（P <= 0.05）。研究了OCAS对胶原蛋白水合在深层的真皮中的影响。 ocas均诱导皮肤脱水，但甘油处理的影响（30分钟和60分钟）更强。所得结果表明，随着治疗时间的增加，甘油和甘油和甘油（TM）溶液均改善猪皮肤的光学清除，使得能够进行研究的深层的真皮区域。在使用的浓度和时间间隔中，甘油比Omnipaque（TM）更有效。然而，Omnipaque（TM）比甘油更有希望，用于在体内应用中的未来应用，因为它是已经批准的药物物质而没有对皮肤结构的任何已知影响。

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