...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes
【24h】

Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes

机译：基于金纳米粒子标记的ssDNA识别和增强探针的新型单步信号放大侧流带超灵敏检测汞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel one-step signal amplified lateral flow strip (SA-LFS) is described for direct ultrasensitive and on- site detection of Hg~(2+) based on the specific recognition system of thymine-Hg~(2+)-thymine using gold nanoparticles (AuNPs) as labeling tags. The signal was also amplified by hybridization induced dual labeling of AuNPs, which increased signal intensity of test line on strips. The presence of both probes on the LFS enabled both sensing and signal amplification to be achieved in a single step. The resulting SA-LFS is capable of rapid and ultrasensitive detection of Hg~(2+) with LODs of 0.005 ppb and 0.0015 ppb by visual observation and quantitative analysis, respectively. At least 40-fold improvement in sensitivity of the traditional LFS was achieved through this signal amplification process. The successful application of the method to the determination of Hg~(2+) in water sample is reported. The proposed signal amplification process may also be applicable to other LFS-based methods.
机译：基于胸腺嘧啶-Hg〜（2 +）-胸腺嘧啶的特异性识别系统，描述了一种新型的一步信号放大侧流条（SA-LFS），用于对Hg〜（2+）的直接超灵敏和现场检测。金纳米颗粒（AuNPs）作为标记标签。杂交诱导的AuNPs双标记也放大了信号，这增加了条带上测试线的信号强度。 LFS上同时存在两个探针，因此可以在单个步骤中完成感测和信号放大。所得的SA-LFS能够通过视觉观察和定量分析分别以0.005 ppb和0.0015 ppb的LOD快速，超灵敏地检测Hg〜（2+）。通过这种信号放大过程，传统LFS的灵敏度至少提高了40倍。报道了该方法在水样中Hg〜（2+）的测定中的成功应用。所提出的信号放大过程也可以适用于其他基于LFS的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号