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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Enzyme biosensor systems based on porous silicon photoluminescence for detection of glucose, urea and heavy metals
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Enzyme biosensor systems based on porous silicon photoluminescence for detection of glucose, urea and heavy metals

机译：基于多孔硅光致发光的酶生物传感器系统，用于检测葡萄糖，尿素和重金属

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摘要

A phenomenon of changes in photoluminescence of porous silicon at variations in medium pH is proposed to be used as a basis for the biosensor system development. The method of conversion of a biochemical signal into an optical one is applied for direct determination of glucose and urea as well as for inhibitory analysis of heavy metal ions. Changes in the quantum yield of porous silicon photoluminescence occur at varying pH of the tested solution due to the enzyme-substrate reaction. When creating the biosensor systems, the enzymes urease and glucose oxidase (GOD) were used as a bioselective material; their optimal concentrations were experimentally determined. It was shown that the photoluminescence intensity of porous silicon increased by 1.7 times when increasing glucose concentration in the GOD-containing reaction medium from 0 to 3.0 mM, and decreased by 1.45 times at the same increase in the urea concentration in the urease-containing reaction medium. The calibration curves of dependence of the biosensor system responses on the substrate concentrations are presented. It is shown that the presence of heavy metal ions (Cu2+, Pb2+, and Cd2+) in the tested solution causes an inhibition of the enzymatic reactions catalyzed by glucose oxidase and urease, which results in a restoration of the photoluminescence quantum yield of porous silicon. It is proposed to use this effect for the inhibitory analysis of heavy metal ions. (C) 2014 Elsevier B.V. All rights reserved.
机译：提出了在介质pH值变化时多孔硅的光致发光变化现象，可作为生物传感器系统开发的基础。将生化信号转换为光学信号的方法适用于直接测定葡萄糖和尿素以及抑制重金属离子的分析。由于酶-底物反应，多孔硅光致发光的量子产率的变化在测试溶液的pH变化时发生。在创建生物传感器系统时，脲酶和葡萄糖氧化酶（GOD）被用作生物选择性材料。通过实验确定它们的最佳浓度。结果表明，当将含GOD的反应介质中的葡萄糖浓度从0增加到3.0 mM时，多孔硅的光致发光强度增加了1.7倍，同时在含脲酶的反应中尿素浓度增加了，多孔硅的光致发光强度降低了1.45倍。中。给出了生物传感器系统响应对底物浓度的依赖性的校准曲线。结果表明，测试溶液中重金属离子（Cu2 +，Pb2 +和Cd2 +）的存在会抑制葡萄糖氧化酶和脲酶催化的酶促反应，从而恢复多孔硅的光致发光量子产率。建议将这种效应用于重金属离子的抑制分析。 （C）2014 Elsevier B.V.保留所有权利。

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