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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Love-wave bacteria-based sensor for the detection of heavy metal toxicity in liquid medium
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Love-wave bacteria-based sensor for the detection of heavy metal toxicity in liquid medium

机译：基于爱波细菌的传感器，用于检测液体介质中的重金属毒性

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摘要

The present work deals with the development of a Love-wave bacteria-based sensor platform for the detection of heavy metals in liquid medium. The acoustic delay-line is inserted in an oscillation loop in order to record the resonance frequency in real-time. A Polydimethylsiloxane (PDMS) chip with a liquid chamber is maintained by pressure above the acoustic wave propagation path.Bacteria (Escherichia coli) were fixed as bioreceptors onto the sensitive surface of the sensor coated with a polyelectrolyte (PE) multilayer using a simple and efficient layer-by-layer (LbL) electrostatic self-assembly procedure. Poly(allylamine hydrochloride) (PAH cation) and poly(styrene sulfonate) (PSS anion) were alternatively deposited so that the strong attraction between oppositely charged polyelectrolytes resulted in the formation of a (PAH-PSS)_n-PAH molecular multilayer. The real-time characterization of PE multilayer and bacteria deposition is based on the measurement of the resonance frequency perturbation due to mass loading during material deposition. Real-time response to various concentrations of cadmium (Cd~(2+)) and mercury (Hg~(2+)) has been investigated. A detection limit as low as 10~(-12)mol/l has been achieved, above which the frequency increases gradually up to 10~(-3)mol/l, after a delay of 60s subsequent to their introduction onto bacterial cell-based biosensors. Beyond a 10~(-3)mol/l a steep drop in frequency was observed. This response has been attributed to changes in viscoelastic properties, related to modifications in bacteria metabolism.
机译：本工作涉及用于检测液体介质中重金属的基于Love-wave细菌的传感器平台的开发。声音延迟线插入振荡回路中，以便实时记录共振频率。通过声波传播路径上方的压力来保持带有液室的聚二甲基硅氧烷（PDMS）芯片。使用简单高效的方法将细菌（大肠杆菌）作为生物受体固定在涂有聚电解质（PE）多层膜的传感器的敏感表面上逐层（LbL）静电自组装过程。交替沉积聚烯丙胺盐酸盐（PAH阳离子）和聚苯乙烯磺酸盐（PSS阴离子），以便带相反电荷的聚电解质之间的强吸引力导致形成（PAH-PSS）_n-PAH分子多层。 PE多层膜和细菌沉积的实时表征基于对材料沉积过程中由于质量负载而引起的共振频率扰动的测量。研究了对各种浓度的镉（Cd〜（2+））和汞（Hg〜（2+））的实时响应。检出限已低至10〜（-12）mol / l，超过此频率后，在将其引入细菌细胞后经过60s的延迟，频率逐渐增加至10〜（-3）mol / l。基于生物传感器。超过10〜（-3）mol / l，观察到频率急剧下降。该反应归因于粘弹性质的变化，与细菌代谢的改变有关。

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