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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >A polycarbonate based surface plasmon resonance sensing cartridge for high sensitivity HBV loop-mediated isothermal amplification
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A polycarbonate based surface plasmon resonance sensing cartridge for high sensitivity HBV loop-mediated isothermal amplification

机译：基于聚碳酸酯的表面等离振子共振传感盒，用于高灵敏度HBV环介导的等温扩增

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摘要

In this study, we report a simple, low-cost surface plasmon resonance (SPR)-sensing cartridge based on a loop-mediated isothermal amplification (LAMP) method for the on-site detection of the hepatitis B virus (HBV). For LAMP detection, a SPR based LAMP sensing system (SPRLAMP) was constructed, including a novel SPRLAMP sensing cartridge integrating a polymethyl methacrylate (PMMA) micro-reactor with a polycarbonate (PC)-based prism coated with a 50. nm Au film.First, we found that the change of refractive index of the bulk solution was approximately 0.0011 refractive index (RI) units after LAMP reaction. The PC-based prism's linearity and thermal responses were compared to those of a traditional glass prism to show that a PC-based prism can be used for SPR measurement. Finally, the HBV template mixed in the 10μl LAMP solution could be detected by SPRLAMP system in 17min even at the detection-limited concentration of 2fg/ml. We also analyzed the correlation coefficients between the initial concentrations of HBV DNA templates and the system response (ΔRU) at varying amplification times to establish an optimal amplification time endpoint of 25min (R ~2=0.98). In conclusion, the LAMP reaction could be detected with the SPRLAMP sensing cartridge based on direct sensing of the bulk refractive index.
机译：在这项研究中，我们报告了一种基于环介导的等温扩增（LAMP）方法的简单，低成本表面等离振子共振（SPR）传感盒，用于现场检测乙型肝炎病毒（HBV）。对于LAMP检测，构建了一个基于SPR的LAMP传感系统（SPRLAMP），包括一个新颖的SPRLAMP传感盒，该盒将聚甲基丙烯酸甲酯（PMMA）微反应器与涂有50 nm金膜的聚碳酸酯（PC）基棱镜结合在一起。首先，我们发现在LAMP反应后，本体溶液的折射率变化约为0.0011折射率（RI）单位。将基于PC的棱镜的线性和热响应与传统玻璃棱镜的线性和热响应进行了比较，表明基于PC的棱镜可用于SPR测量。最后，即使检测极限浓度为2fg / ml，SPRLAMP系统也可以在17min内检测到混入10μlLAMP溶液中的HBV模板。我们还分析了在不同的扩增时间下HBV DNA模板的初始浓度与系统响应（ΔRU）之间的相关系数，以建立最佳的25min扩增时间终点（R〜2 = 0.98）。总而言之，可以使用SPRLAMP传感盒基于对大折射率的直接传感来检测LAMP反应。

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