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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Ultrasensitive sensing platform for platelet-derived growth factor BB detection based on layered molybdenum selenide-graphene composites and Exonuclease III assisted signal amplification
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Ultrasensitive sensing platform for platelet-derived growth factor BB detection based on layered molybdenum selenide-graphene composites and Exonuclease III assisted signal amplification

机译：基于层状硒化钼-石墨烯复合材料和核酸外切酶III辅助信号放大的血小板衍生生长因子BB检测超灵敏传感平台

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摘要

A highly sensitive and ultrasensitive electrochemical aptasensor for platelet-derived growth factor BB (PDGF-BB) detection is fabricated based on layered molybdenum selenide-graphene (MoSe2-Gr) composites and Exonuclease III (Exo III)-aided signal amplification. MoSe2-Gr is prepared by a simple hydrothermal method and used as a promising sensing platform. Exo III has a specifical exo-deoxyribonuclease activity for duplex DNAs in the direction from 3' to 5' terminus, however its activity is limited on the duplex DNAs with more than 4 mismatched terminal bases at 3' ends. Herein, aptamer and complementary DNA (cDNA) sequences are designed with four thymine bases on 3' ends. In the presence of target protein, the aptamer associates with it and facilitates the formation of duplex DNA between cDNA and signal DNA. The duplex DNA then is digested by Exo III and releases cDNA, which hybridizes with signal DNA to perform a new cleavage process. Nevertheless, in the absence of target protein, the aptamer hybridizes with cDNA will inhibit the Exo III-assisted nucleotides cleavage. The signal DNA then hybridizes with capture DNA on the electrode. Subsequently, horse radish peroxidase is fixed on electrode by avidin-biotin reaction and then catalyzes hydrogen peroxide and hydroquinone to produce electrochemical response. Therefore, a bridge can be established between the concentration of target protein and the degree of the attenuation of the obtained signal, providing a quantitative measure of target protein with a broad detection range of 0.0001-1 nM and a detection limit of 20 fM. (C) 2015 Elsevier B.V. All rights reserved.
机译：基于层状硒化钼-石墨烯（MoSe2-Gr）复合材料和核酸外切酶III（Exo III）辅助的信号放大技术，制造了用于血小板衍生生长因子BB（PDGF-BB）检测的高度灵敏和超灵敏的电化学适体传感器。 MoSe2-Gr是通过简单的水热法制备的，可用作有希望的传感平台。 Exo III在3'到5'末端对双链DNA具有特定的exo-deoxy-ribonuclease活性，但是其活性仅限于在3'末端具有4个以上错配末端碱基的双链DNA。在本文中，设计适体和互补DNA（cDNA）序列，在3'端具有四个胸腺嘧啶碱基。在靶蛋白存在下，适体与其结合并促进cDNA与信号DNA之间双链DNA的形成。然后，双链体DNA被Exo III消化并释放cDNA，该cDNA与信号DNA杂交以执行新的切割过程。然而，在没有靶蛋白的情况下，与cDNA杂交的适体将抑制Exo III辅助的核苷酸切割。然后，信号DNA与电极上的捕获DNA杂交。随后，辣根过氧化物酶通过亲和素-生物素反应固定在电极上，然后催化过氧化氢和氢醌产生电化学反应。因此，可以在目标蛋白质的浓度和获得的信号的衰减程度之间建立桥梁，从而提供定量检测目标蛋白质的方法，检测范围为0.0001-1 nM，检测极限为20 fM。 （C）2015 Elsevier B.V.保留所有权利。

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