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首页> 外文期刊>Academic radiology >The effects of magnetically labeled rat spleen-originated endothelial progenitor cells on growth of glioma in vivo an experimental study.
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The effects of magnetically labeled rat spleen-originated endothelial progenitor cells on growth of glioma in vivo an experimental study.

机译：体内磁性标记大鼠脾源性内皮祖细胞对神经胶质瘤生长的影响的实验研究。

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RATIONALE AND OBJECTIVES: The aim of this study was to investigate the effects of exogenous endothelial progenitor cells (EPCs) on the growth and invasiveness of glioma in vivo to provide an experimental basis for the value and safety of using magnetically labeled EPCs as target vectors to detect early infiltration of glioma. MATERIALS AND METHODS: EPCs were collected from the spleens of healthy Sprague-Dawley rats, made EPCs conditioned medium after identification. Four models of Sprague-Dawley rat glioma (60 rats in total) were established as a control and three experimental groups (group A, B, and C). In the control group, orthotopic transplantation of C6 glioma cells was performed. Compared to the control group, EPCs conditioned medium was added in group A and P7228-labeled EPCs were added in group B. In group C, P7228-labeled EPCs were transplanted via the tail vein. Magnetic resonance imaging and perfusion-weighted imaging were performed on several days. Tumor microvascular density and vascular endothelial growth factor expression were determined through immunohistochemistry. RESULTS: In group C, hypointense areas were detected at the periphery of the tumor on the first day after transplantation of EPCs, and more hypointense areas were found inside the tumor over time. Tumor size in all four groups developed significantly with increasing time (P < .01), but there was no marked difference among these groups at the same time (P > .05). No remarkable differences in microvascular density and cells positive for vascular endothelial growth factor were found at the same time among the four groups (P > .05). CONCLUSIONS: Both magnetic resonance imaging and immunohistochemical findings confirmed that exogenous EPCs could not affect the biologic behavior of C6 glioma cells in vivo through a paracrine effect or by direct cellular interaction. Therefore, exogenous EPCs could not exert significant promoting effects on glioma growth.
机译：理由和目的：这项研究的目的是研究外源性内皮祖细胞（EPC）对体内神经胶质瘤的生长和侵袭性的影响，从而为使用磁性标记EPC作为靶标载体的价值和安全性提供实验基础。检测神经胶质瘤的早期浸润。材料与方法：从健康的Sprague-Dawley大鼠脾脏中收集EPCs，经鉴定后制成EPCs条件培养基。建立了四种Sprague-Dawley大鼠神经胶质瘤模型（总共60只大鼠）作为对照组，并建立了三个实验组（A，B和C组）。在对照组中，进行了C6神经胶质瘤细胞的原位移植。与对照组相比，在A组中添加了EPCs条件培养基，在B组中添加了P7228标记的EPC。在C组中，P7228标记的EPCs通过尾静脉移植。几天进行了磁共振成像和灌注加权成像。通过免疫组织化学测定肿瘤微血管密度和血管内皮生长因子表达。结果：C组在EPCs移植后的第一天，在肿瘤周围发现了低血脂区域，随着时间的推移，在肿瘤内部发现了更多的低血脂区域。随着时间的延长，所有四个组的肿瘤大小均显着发展（P <.01），但同时这些组之间的肿瘤大小无显着差异（P> .05）。在四组中，同时没有发现微血管密度和血管内皮生长因子阳性细胞的显着差异（P> .05）。结论：磁共振成像和免疫组化研究结果均证实，外源性EPC不能通过旁分泌作用或直接的细胞相互作用影响体内C6胶质瘤细胞的生物学行为。因此，外源性EPC不能对神经胶质瘤的生长产生明显的促进作用。

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