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首页> 外文期刊>Academic radiology >In vivo lipid profiling using proton magnetic resonance spectroscopy in an experimental liver fibrosis model.
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In vivo lipid profiling using proton magnetic resonance spectroscopy in an experimental liver fibrosis model.

机译：在实验性肝纤维化模型中使用质子磁共振波谱对体内脂质进行分析。

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RATIONALE AND OBJECTIVES: The aim of this study was to characterize early hepatic lipid changes in an experimental model of liver fibrosis using proton ((1)H) magnetic resonance spectroscopy (MRS) at high magnetic field in vivo. MATERIALS AND METHODS: Liver fibrosis was induced in 12 Sprague-Dawley rats by twice-weekly carbon tetrachloride (CCl(4)) administration up to 4 weeks. Eight normal rats were used as controls. Single-voxel (1)H MRS experiments were performed at 7 Tesla to measure signal integrals of various lipid peaks including -CH(3), (-CH(2)-)(n), -CH(2)-C=C-CH(2)-, =C-CH(2)-C= and -CH=CH- at 0.9, 1.3, 2.0, 2.8, and 5.3 ppm, respectively, and peak from choline-containing compounds (CCC) at 3.2 ppm. Total lipid, total saturated fatty acid, total unsaturated fatty acid, total unsaturated bond, polyunsaturated bond, and CCC indices were quantified. RESULTS: Significant increases (P < .01) in total lipid and total saturated fatty acid indices were found in animals with CCl(4)-induced fibrosis as compared with normal animals. In addition, total unsaturated bond and polyunsaturated bond indices of animals at 4 weeks after CCl(4) insult were significantly higher than (P < .01 and P < .05, respectively) those of normal animals and animals at 2 weeks following insu whereas there was only significant increase (P < .01) in total unsaturated fatty acid index in animals with 4-week CCl(4) insult as compared with normal animals. CONCLUSION: The hepatic lipid changes in CCl(4)-induced experimental fibrosis model were documented in vivo and longitudinally using (1)H MRS at 7 Tesla. The experimental findings suggested that total saturated fatty acid increase contributed mainly to the total lipid increase in animals with CCl(4) insult. This study also demonstrated the potential value of high field MRS to resolve lipid composition and alterations in liver fibrosis.
机译：理由和目的：这项研究的目的是在体内高磁场下使用质子（（1）H）磁共振波谱（MRS）在肝纤维化实验模型中表征早期肝脂质变化。材料与方法：每周两次两次四氯化碳（CCl（4））给药直至12周，在12只Sprague-Dawley大鼠中诱发肝纤维化。八只正常大鼠用作对照。在7 Tesla下进行单素（1）H MRS实验以测量各种脂质峰的信号积分，包括-CH（3），（-CH（2）-）（n），-CH（2）-C = C -CH（2）-，= C-CH（2）-C =和-CH = CH-分别为0.9、1.3、2.0、2.8和5.3 ppm，并且来自含胆碱化合物（CCC）的峰为3.2 ppm。定量总脂质，总饱和脂肪酸，总不饱和脂肪酸，总不饱和键，多不饱和键和CCC指数。结果：与正常动物相比，CCl（4）诱导的纤维化动物的总脂质和总饱和脂肪酸指数显着增加（P <.01）。此外，CCl（4）损伤后4周时动物的总不饱和键和多不饱和键指数显着高于正常动物和损伤后2周时的动物的不饱和键和多不饱和键指数（分别为P <.01和P <.05）;然而，与正常动物相比，遭受4周CCl（4）侵害的动物的总不饱和脂肪酸指数仅显着增加（P <.01）。结论：在体内和纵向使用（1）H MRS在7 Tesla下记录了CCl（4）诱导的实验性纤维化模型中肝脂质的变化。实验结果表明，总饱和脂肪酸增加主要是造成CCl（4）污染的动物体内总脂质增加的原因。这项研究还证明了高场MRS解决脂质成分和肝纤维化改变的潜在价值。

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