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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >A novel enzyme biosensor for glucose based on rhodanine derivative chemiluminescence system and mesoporous hollow silica microspheres receptor
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A novel enzyme biosensor for glucose based on rhodanine derivative chemiluminescence system and mesoporous hollow silica microspheres receptor

机译：基于若丹宁衍生物化学发光系统和介孔中空二氧化硅微球受体的新型葡萄糖生物酶传感器

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In this work, hollow silica microspheres (HSMs), which have hollow interiors and nanoporous shells, were used in the immobilization of enzyme to establish a novel chemiluminescence biosensor. The immobilization behavior of enzyme in HSMs with different pore sizes has been studied. The results revealed that the pore size and the surface area of HSMs play important roles in their immobilization performance. Compared with traditional methods, this immobilization method not only provides tunable and consistent pore system and restricted microspaces for enzyme immobilization, but also exhibits a larger immobilization capacity and a higher adsorption rate. A rhodanine derivative-KMnO4-HCl-H2O2 was used to replace traditional chemiluminescence system (luminol-horseradish peroxidase-H2O2) to reach the purpose of high sensitivity, simple operation and pH expansion for chemiluminescence biosensor. The linear range of this novel method is 7.72 x 10(-6) to 2.54 x 10(-2) mol L-1 (r = 0.9994). The detection limit is 8.0 x 10(-7) mol L. The Michaelis-Menten constant of glucose oxidase was 0.3 mmol L-1. With glucose oxidase as a test enzyme, the proposed method gave an accurate and satisfactory result once applied to the determination of glucose in four different human serum samples. (C) 2010 Elsevier B.V. All rights reserved.
机译：在这项工作中，具有中空内部和纳米孔壳的中空二氧化硅微球（HSM）被用于固定酶，从而建立了一种新型的化学发光生物传感器。研究了酶在不同孔径的HSM中的固定化行为。结果表明，HSM的孔径和表面积在其固定性能中起重要作用。与传统方法相比，该固定方法不仅为酶固定提供了可调节且一致的孔系统和有限的微空间，而且具有更大的固定能力和更高的吸附率。罗丹宁衍生物KMnO4-HCl-H2O2代替了传统的化学发光系统（鲁米诺-辣根过氧化物酶-H2O2），达到了化学发光生物传感器的高灵敏度，操作简单和pH扩展的目的。这种新方法的线性范围是7.72 x 10（-6）到2.54 x 10（-2）mol L-1（r = 0.9994）。检测极限为8.0 x 10（-7）molL。葡萄糖氧化酶的Michaelis-Menten常数为0.3 mmol L-1。以葡萄糖氧化酶为测试酶，该方法一旦用于四种不同人血清样品中葡萄糖的测定，即可得到准确而令人满意的结果。 （C）2010 Elsevier B.V.保留所有权利。

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