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首页> 外文期刊>Acta Chromatographica >BMD-TLC-a useful separation technique for quantitative analysis of arbutin and hydroquinone in herbal drugs
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BMD-TLC-a useful separation technique for quantitative analysis of arbutin and hydroquinone in herbal drugs

机译：BMD-TLC-一种有用的分离技术，用于定量分析草药中的熊果苷和对苯二酚

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摘要

A three-step, bivariant multiple-development TLC technique (BMD-TLC) was used in the quantitative analysis of arbutin and hydroquinone (aglycone of arbutin) in commercially available herbal drugs used in kidney disorders. The separation was carried out on TLC Si60 F254s plates at a distance of 10 cm. The first step was the separation of analyzed compounds from ballasts at a distance of 1 cm in methanol; the second step was the separation of arbutin at a distance of 5.5 cm in an ethyl acetate-methanol-water (77:13:10) solvent mixture; and the third step was the separation of hydroquinone at a distance of 4.5 cm in n-heptane-ethyl acetate (1:1) solvent mixture. At each step, the ballast zones were cut away. The densitometric quantification of arbutin and hydroquinone was performed at 285 nm. The linearity, precision, repeatability, LOD, and LOQ were determined for the method. In addition, the influence of chromatogram scanning technique on the results of quantitative analysis was determined. It is pointed out that the optimized MD-TLC separation method can be used in the qualitative and quantitative analysis of arbutin and hydroquinone both in raw plant matrices and in herbal drugs.
机译：在用于肾脏疾病的市售草药中，熊果苷和对苯二酚（熊果苷的糖苷配基）的定量分析采用了三步法，双变量，多重开发的TLC技术（BMD-TLC）。在TLC Si60 F254s板上以10 cm的距离进行分离。第一步是在甲醇中1 cm处从压载物中分离出被分析的化合物;第二步是在乙酸乙酯-甲醇-水（77:13:10）溶剂混合物中以5.5 cm的距离分离熊果苷。第三步是在正庚烷-乙酸乙酯（1：1）溶剂混合物中分离4.5 cm处的对苯二酚。在每个步骤中，都将镇流器区域切掉。在285nm下进行熊果苷和对苯二酚的光密度定量。确定了该方法的线性，精密度，可重复性，LOD和LOQ。另外，确定了色谱扫描技术对定量分析结果的影响。指出优化的MD-TLC分离方法可用于植物原料和草药中熊果苷和对苯二酚的定性和定量分析。

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