• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >A chimeric epidermal growth factor with fibrin affinity promotes repair of injured keratinocyte sheets.
【24h】

A chimeric epidermal growth factor with fibrin affinity promotes repair of injured keratinocyte sheets.

机译：具有纤维蛋白亲和力的嵌合表皮生长因子促进受损角质形成细胞片的修复。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of the present study is to create a novel chimeric protein of epidermal growth factor (EGF) with fibrin affinity and demonstrate its potential for repairing injured tissues by immobilization to fibrin. The chimeric protein (FBD-EGF) was produced by the fusion of the fibronectin fibrin-binding domain (FBD) to EGF. It showed dose-dependent binding to fibrin and its binding was stable for at least 7days, while native EGF showed little affinity. FBD-EGF promoted the growth of fibroblasts and keratinocytes in the fibrin-bound state as well as in the soluble state. Its activity was further studied in a keratinocyte culture system in which fibrin was exposed upon injury of cell sheets. Fibrin-bound FBD-EGF promoted growth of the sheets over the injured area at a significantly faster rate (approximately eightfold) than native EGF (p<0.01). Wounds 2mm wide were closed in 7-9days. This repair process was inhibited by anti-EGF. Keratinocytes proliferated more extensively in the leading edges of sheets contacting fibrin with FBD-EGF, approximately 1.7-fold more than in the adjacent regions. These results imply that the stable binding of chimeric EGF to fibrin is effective for the repair of injured keratinocyte sheets, suggesting a potential use in tissue engineering.
机译：本研究的目的是创建具有纤维蛋白亲和力的新型表皮生长因子（EGF）嵌合蛋白，并证明其通过固定在纤维蛋白上修复受损组织的潜力。嵌合蛋白（FBD-EGF）是通过纤连蛋白纤维蛋白结合结构域（FBD）与EGF融合而产生的。它显示出对血纤蛋白的剂量依赖性结合，并且其结合至少持续7天是稳定的，而天然EGF却显示出很少的亲和力。 FBD-EGF以纤维蛋白结合状态和可溶状态促进成纤维细胞和角质形成细胞的生长。在角质形成细胞培养系统中进一步研究了它的活性，在该系统中，在细胞片受损时血纤蛋白被暴露出来。纤维蛋白结合的FBD-EGF以比天然EGF显着更快的速度（约8倍）促进了受伤区域的片层生长（p <0.01）。在7-9天之内关闭2mm宽的伤口。该修复过程被抗EGF抑制。角质形成细胞在纤维蛋白与FBD-EGF接触的薄片的前缘更广泛地增殖，比相邻区域多1.7倍。这些结果暗示嵌合EGF与纤维蛋白的稳定结合对于修复受损的角质形成细胞片是有效的，表明在组织工程中的潜在用途。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号