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首页> 外文期刊>Acta biomaterialia >Direct effect of alginate purification on the survival of islets immobilized in alginate-based microcapsules.
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Direct effect of alginate purification on the survival of islets immobilized in alginate-based microcapsules.

机译：海藻酸盐纯化对固定在基于海藻酸盐的微胶囊中的胰岛存活的直接影响。

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Alginate purification has been shown to decrease the host immune response to implanted alginate-based microcapsules, but the direct effect of contaminants on islet cell survival remains unknown. Wistar rat islets were immobilized in calcium alginate beads made with crude vs. purified alginate and then incubated in CMRL culture medium. Islet survival was evaluated at 1, 4, 7, 14 and 27 days post-encapsulation. Islet viability was investigated using a dual staining assay (propidium iodide and orange acridine). The islet cell necrosis and the proportion of apoptotic cells were quantified under optical microscopy and with a TUNEL assay, respectively. Islets immobilized in purified alginate were more viable, and had fewer necrotic centers, a smaller area of central necrosis and a lower number of apoptotic cells. At day 14 and 27 post-encapsulation, respectively, 48% and 23% of islets were viable with purified alginate vs. 18% and 8% with crude alginate (p<0.05). At day 14, the surface area of central necrosis and the number of necrotic islets were more important with the impure alginate (65% vs. 45% and 73% vs. 53%, respectively; p<0.05). We conclude that alginate purification improves the survival of islets that are immobilized in alginate-based microcapsules. These findings indicate that caution should be taken in the interpretation of in vivo experiments, as the results could be explained by either a direct effect on islet survival or a modification of the host reaction, or both. Moreover, it suggests that the effect on islet viability should be assessed during the development of biomaterials for cell encapsulation.
机译：已显示藻酸盐纯化可降低宿主对植入的基于藻酸盐的微胶囊的免疫反应，但污染物对胰岛细胞存活的直接影响仍然未知。将Wistar大鼠胰岛固定在用粗藻酸盐与纯化藻酸盐制成的藻酸盐钙珠中，然后在CMRL培养基中孵育。在封装后1、4、7、14和27天评估胰岛存活。使用双重染色测定法（碘化丙啶和橙a啶）研究胰岛的生存力。胰岛细胞坏死和凋亡细胞的比例分别在光学显微镜和TUNEL分析中进行定量。固定在纯化藻酸盐中的胰岛更可行，坏死中心更少，中央坏死面积更小，凋亡细胞数量更少。包囊后第14天和第27天，分别使用纯化的藻酸盐存活的胰岛为48％和23％，而使用粗藻酸盐存活的胰岛分别为18％和8％（p <0.05）。在第14天，不纯藻酸盐对中心坏死的表面积和坏死胰岛的数量更为重要（分别为65％vs. 45％和73％vs. 53％; p <0.05）。我们得出的结论是，藻酸盐纯化可提高固定在基于藻酸盐的微胶囊中的胰岛的存活率。这些发现表明，在体内实验的解释中应谨慎行事，因为其结果可以通过对胰岛存活率的直接影响或宿主反应的改变或两者来解释。此外，这表明在开发用于细胞包封的生物材料的过程中，应评估对胰岛活力的影响。

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