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首页> 外文期刊>Acta biomaterialia >In vitro integration of human skin dermis with porous cationic hydrogels.
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In vitro integration of human skin dermis with porous cationic hydrogels.

机译：人皮肤真皮与多孔阳离子水凝胶的体外整合。

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摘要

Porous poly(DMAA-co-AMTAC) hydrogels, fabricated using the inverted colloid crystal method, were used to observe their integration with human skin. Full thickness human breast skin explants discarded from surgeries were cultured for up to 10days at the air-liquid interface using a Transwell culture system. Cylindrical, disk- or other shaped hydrogels were placed inside the skin explants fitting punctures produced by punch biopsies or scalpels and full section histological analysis of the skin explants with the inserted hydrogel was then performed. In addition, separated hydrogels were cultured up to 7days with human fibroblasts. The results indicate that poly(DMAA-co-AMTAC) hydrogels induce substantial extracellular matrix material deposition, maintain dermal integrity in the contact areas with the skin and permit dermal fibers to integrate into the hydrogel pores. Different types of cells remaining in the explants migrated into the hydrogels pores, including red blood cells. Fibroblasts adhered to and colonized separately cultured hydrogels. We plan to use this type of soft material as an interface to permit skin integration with percutaneous devices in contact with skin.
机译：使用倒胶体晶体法制备的多孔聚（DMAA-co-AMTAC）水凝胶用于观察其与人体皮肤的整合。使用Transwell培养系统，将从手术中丢弃的全厚度人乳房外植体在气液界面上培养长达10天。将圆柱状，圆盘状或其他形状的水凝胶放置在皮肤外植体内部，以适应由打孔活组织检查或手术刀产生的穿刺，然后用插入的水凝胶对皮肤外植体进行完整切片的组织学分析。此外，将分离的水凝胶与人成纤维细胞培养长达7天。结果表明，聚（DMAA-co-AMTAC）水凝胶可诱导大量细胞外基质物质沉积，维持与皮肤接触区域的真皮完整性，并使真皮纤维整合到水凝胶孔中。外植体中残留的不同类型的细胞迁移到水凝胶的孔中，包括红细胞。成纤维细胞粘附并定殖在单独培养的水凝胶上。我们计划将这种类型的软材料用作界面，以使皮肤与与皮肤接触的经皮设备整合。

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